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Molecular characterization of Vigna radiata (L.) Wilczek genotypes based on nuclear ribosomal DNA and RAPD polymorphism.

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Title Molecular characterization of Vigna radiata (L.) Wilczek genotypes based on nuclear ribosomal DNA and RAPD polymorphism.
Not Available
 
Creator Raturi, A.,
Singh, S.K.,
Sharma V.
Pathak, R.
 
Subject DNA bar coding Genetic diversity Mungbean RAPD rDNA diversity Vigna radiata
 
Description Not Available
Mungbean germplasm characterization, evaluation
and improvement are fundamentally based on morpho-
agronomic traits. The lack of break-through in
mungbean production has been due to non-availability of
genetic variability for high yield potential. Forty-four
genotypes of mungbean [Vigna radiata (L.)Wilczek] were
subjected to random amplified polymorphic DNA (RAPD)
analysis to assess the genetic diversity and relationships
among the genotypes. Multilocus genotyping by twelve
RAPD primers generated 166 markers and detected an
average of intraspecific variation amounting to 82% polymorphism
in banding patterns. Dendrogram obtained from
cluster analysis delineated all the 44 genotypes into six
clusters. Higher values of Nei’s gene diversity (h) and
Shannon information index (i) and genetic distance analysis
validate existence of wide genetic diversity among
mungbean genotypes tested. Besides internal transcribed
spacer (ITS) length variations, single nucleotide polymorphisms
(SNPs) and insertions/deletions (INDELS) were
detected at number of sites in nuclear rDNA region and the
sequences of representatives of each sub-cluster and all
distinct genotypes have been submitted to NCBI database
and assigned Gen accession numbers HQ 148136-148147.
Multiple sequence alignment revealed further lineages of
distinct genotypes with main RAPD clusters. The measures
of relative genetic distances among the genotypes of
mungbean did not completely correlate the geographical
places of their development. The homogeneous phenotypic
markers proved insufficient in exhibiting genetic divergence
among mungbean genotypes studied. RMG-62,
RMG-976, and NDM-56 have been identified as potential
source of parents for crop improvement. RAPD primers,
OPA-9 and OPA-2 as polymorphic genetic markers and
number of pods/plant and number of seeds/plant as
dependable phenotypic markers have been identified for
improving yield potentials. This genetic diversity will be of
significance in developing intraspecific crosses in mungbean
crop improvement programme
CSIR
 
Date 2018-11-14T12:09:04Z
2018-11-14T12:09:04Z
2012-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/Publication/handle/123456789/11343
 
Language English
 
Relation Not Available;
 
Publisher Springer