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Seasonal and micro-environmental factors controlling clonal propagation of mature trees of marwar teak [Tecomella undulata (Sm.) Seem]

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Title Seasonal and micro-environmental factors controlling clonal propagation of mature trees of marwar teak [Tecomella undulata (Sm.) Seem]
Not Available
 
Creator Sidhika Chhajer • Rajwant K. Kalia
 
Subject groforestry tree Axillary bud multiplication Desert teak Genetic fidelity Micropropagation Rohida
 
Description Not Available
A simple method has been developed for clonal
propagation of mature trees of Tecomella undulata (Sm.)
Seem, a medicinally important deciduous timber tree of hot
arid regions, via multiple shoot proliferation from axillary
buds after examining the role of season influences and
physico–chemical conditions on micropropagation. Spring
season (March–April) was the best period for contamination free establishment of explants and maximum sprouting
of healthy axillary buds. Shoots proliferated directly from
the explant nodes cultured on Murashige and Skoog’s
medium containing cytokinins, BAP supporting better
growth compared to kinetin during shoot induction as well
as multiplication phase. Cytokinin concentration influenced
the bud induction frequency and optimal response of 2.6
buds per explant was achieved in 86.66% explants on
media supplemented with 10 lM BAP. Stunted shoot buds
with excessive callus were observed when cytokinin concentration was increased beyond optimal levels. Ascorbic
acid (50 mg/l), arginine and citric acid (25 mg/l each) were
added to proliferation and multiplication media for reducing callus proliferation and better shoot growth. Among the
media (B5, MS, NN, WPM and SH) tested, SH was best for
shoot multiplication. Shoot cultures were multiplied by
regular subculture of axillary shoots on SH medium containing 5.0 lM each of BAP and kinetin. Shoots produced
roots when cultured on 9 SH medium ? 10 lM IBA.
Regenerated plantlets were successfully transferred to field after hardening and acclimatization. Genetic homogeneity
of tissue culture raised plants was confirmed by generation
of monomorphic DNA fragments with Start codon targeted
and intersimple sequence repeat (ISSR) markers.
Not Available
 
Date 2018-11-14T09:32:48Z
2018-11-14T09:32:48Z
2017-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/Publication/handle/123456789/11196
 
Language English
 
Relation Not Available;
 
Publisher Not Available