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Grain protein estimation and SDS-PAGE profiling of six important arid legumes

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Title Grain protein estimation and SDS-PAGE profiling of six important arid legumes
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Creator A.K. Jukanti, H.R. Dagla, P. Kalwani, D. Goswami, J.M. Upendra, R.K. Kalia and R.K. Bhatt
 
Subject Arid legumes, Grain protein, Protein profile, Similarity index.
 
Description Not Available
Grain protein content and protein profiles of six arid grain legumes belonging to three different tribes were studied. Average
grain protein (per cent) of horse gram, cowpea, moth bean, guar, chickpea and green gram was 20.29, 22.8, 22.6, 20.6,
21.82, and 20.40 respectively. All the six arid legumes were clearly differentiated based on the protein banding patterns
generated through SDS-PAGE. The molecular weight range of proteins in different legumes was observed to be ~16 to
~250kD. Interestingly, in chickpea, moth bean and horse gram proteins >100kD were not mostly observed. Further, distinct
proteins were observed indicating inter-varietal differences in horse gram (CRHG-19), cowpea (RC-101) and guar (HG-2-
20), which could be useful in varietal identification. The different legumes were broadly clustered in to four clusters.
Further, chickpea (Cicereae), green gram and cowpea (Phaseoleae) were clustered together. The similarity indexes of
varieties and individual crops ranged from 0.667 to 0.950 and 0.425 to 0.821 respectively. Though the six legumes belong
to Leguminosae they have distinct protein profiles which could be useful in species/varietal identification.
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Date 2018-11-14T09:31:20Z
2018-11-14T09:31:20Z
2017-04-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/Publication/handle/123456789/11194
 
Language English
 
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Publisher Not Available