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Genetic diversity in sewan grass (Lasiurus sindicus Henr.) in the hot arid ecosystem of Thar Desert of Rajasthan, India

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Title Genetic diversity in sewan grass (Lasiurus sindicus Henr.) in the hot arid ecosystem of Thar Desert of Rajasthan, India
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Creator R. Sharma, M. P. Rajora, R. Dadheech, R. K. Bhatt and R. K. Kalia
 
Subject Arid ecosystem, Desert grass, Genetic diversity, Sewan grass
 
Description Not Available
Aim : Sewan grass (Lasiurus sindicus Henr.), considered as the “King of Desert Grasses”, is a dominant
grass species of hot arid ecosystem of Great Indian Desert, covering Western Rajasthan and parts of
Pakistan. This grass is extremely drought resistant and thrives even in areas receiving very low rainfall (100
to 150 mm) annually under extreme temperatures ranging from -3 to 50 ºC. The present study was
undertaken to analyze the extent of genetic variability existing among the L. sindicus germplasm collected
from Bikaner, Barmer and Jaisalmer, the diversity rich districts of hyper-arid Rajasthan, using ISSR and
RAPD markers.
Methodology : Twenty seven genotypes of L. sindicus were collected from Jaisalmer (10 accessions),
Barmer (9 accessions) and Bikaner (7 accessions) and 1 old collection maintained at CAZRI, Jodhpur,
Rajasthan was used in this study. A total of 18 RAPD and 14 ISSR markers were screened of which 12
RAPD and 10 ISSR primers amplified distinct and scorable fragments. Data analysis was performed using
NTSYS-pc, SIMQUAL, Genalex 6.5 and POPGENE version 1.32 programs, and dendrograms were
generated using unweighted pair group method
for arithmetic mean (UPGMA).
Results : The comparative analysis of data
showed that RAPD markers were better than
ISSR with regard to polymorphism detection, as
they detected 90% polymorphism in
comparison to 74% for ISSR markers. The
values of average number of polymorphic
fragments per assay, polymorphism information
content (PIC) and discriminatory power (Dj)
were more for RAPD (5.83, 0.222 and 0.78
respectively) than for ISSR (5.7, 0.138 and
0.605 respectively) markers. The UPGMA
clustering was not conspicuous under the
influence of high within region diversity,
however, accessions collected from same
region tended to cluster together. Genetic
similarity values obtained from Jaccard's
coefficient using combined data of both the
marker systems were between 0.58 and 0.74.
Interpretation : The results indicated the
existence of wide genetic variability within and
among regions in this species which can be
used for germplasm conservation and
improvement.
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Date 2018-11-14T11:38:31Z
2018-11-14T11:38:31Z
2017-05-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/Publication/handle/123456789/11294
 
Language English
 
Relation Not Available;
 
Publisher Not Available