Record Details

Evaluation of genetic homogeneity of in vitro-raised plants of Tecomella undulata (Sm.) Seem. using molecular markers

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Evaluation of genetic homogeneity of in vitro-raised plants of Tecomella undulata (Sm.) Seem. using molecular markers
Not Available
 
Creator Sidhika Chhajer & Rajwant K. Kalia
 
Subject Genetic fidelity testing . In vitro cultures . Micropropagation . Molecular markers . Nodal segments . Rohida
 
Description Not Available
Tecomella undulata (Sm.) Seem (family
Bignoniaceae) is an economically and pharmaceutically important timber tree of arid regions of India. Overexploitation of
natural stands coupled with minimal conservation and reforestation efforts has led to its incorporation in list of endangered
species. This monotypic genus can be propagated only through
seeds as no methods are available for its vegetative propagation.
Therefore, protocol for multiplication of T. undulata via direct
regeneration using nodal segments from mature trees has been
standardized. Authentication of genetic homogeneity of these in
vitro-raised plants is necessary for commercial-scale application
of the developed micropropagation protocol. PCR-based molecular markers which have emerged as simple, fast, reliable,
and labor-effective tools for testing the genetic homogeneity of
in vitro-raised plants were used in the present study. Arbitrary
(random amplified polymorphic DNA, RAPD), semi-arbitrary
(inter-simple sequence repeat, ISSR; start codon targeted
(SCoT) polymorphism), and sequence-based (simple sequence
repeat, SSR) markers were used. DNA samples of shoots maintained in vitro for 2 years collected after every 4 subculture
cycles (of 3 weeks each) and field-transferred plantlets were
compared with the mother tree DNA using 131 primers (25
each of RAPD, ISSR, SCoT and 56 SSR). Scorable unambiguous and reproducible DNA fragments were produced by 77
(21 RAPD, 20 ISSR, 22 SCoT and 14 SSR) primers. A total of 71, 93, 94, and 42 distinct and scorable DNA fragments were
produced by RAPD, ISSR, SCoT, and SSR primers respectively with an average of 3.38, 4.65, 4.27, and 3.0 DNA fragments
per primer. The true-to-type nature of the in vitro-raised plants
of T. undulata undergoing up to 32 subculture passages over a
period of approximately 2 years was authenticated by monomorphic DNA fragments amplified with all primer combinations. Therefore, the developed micropropagation protocol can
be safely used on a commercial scale for multiplying
T. undulata plants.
Not Available
 
Date 2018-11-14T11:39:18Z
2018-11-14T11:39:18Z
2016-09-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/Publication/handle/123456789/11298
 
Language English
 
Relation Not Available;
 
Publisher Not Available