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Quantitative determination of guggulsterone in existing natural populations of Commiphora wightii (Arn.) Bhandari for identification of germplasm having higher guggulsterone content

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Title Quantitative determination of guggulsterone in existing natural populations of Commiphora wightii (Arn.) Bhandari for identification of germplasm having higher guggulsterone content
Not Available
 
Creator Alpana Kulhari & Arun Sheorayan & Ashok Chaudhury & Susheel Sarkar & Rajwant K. Kalia
 
Subject Biochemical characterization . Burseraceae . Endangered species . Guggulsterone . Oleo-gum resin
 
Description Not Available
Guggulsterone is an aromatic steroidal ketonic compound obtained from vertical rein ducts and canals of bark of
Commiphora wightii (Arn.) Bhandari (Family - Burseraceae).
Owing to its multifarious medicinal and therapeutic values as
well as its various other significant bioactivities, guggulsterone
has high demand in pharmaceutical, perfumery and incense
industries. More and more pharmaceutical and perfumery industries are showing interest in guggulsterone, therefore, there is a
need for its quantitative determination in existing natural populations of C. wightii. Identification of elite germplasm having
higher guggulsterone content can be multiplied through conventional or biotechnological means. In the present study an effort
was made to estimate two isoforms of guggulsterone i.e. E and Z
guggulsterone in raw exudates of 75 accessions of C. wightii
collected from three states of North-western India viz. Rajasthan
(19 districts), Haryana (4 districts) and Gujarat (3 districts).
Extracted steroid rich fraction from stem samples was fractionated using reverse-phase preparative High Performance Liquid
Chromatography (HPLC) coupled with UV/VIS detector operating at wavelength of 250 nm. HPLC analysis of stem
samples of wild as well as cultivated plants showed that the
concentration of E and Z isomers as well as total guggulsterone
was highest in Rajasthan, as compared to Haryana and Gujarat
states. Highest concentration of E guggulsterone (487.45 μg/g)
and Z guggulsterone (487.68 μg/g) was found in samples collected from Devikot (Jaisalmer) and Palana (Bikaner) respectively, the two hyper-arid regions of Rajasthan, India. Quantitative
assay was presented on the basis of calibration curve obtained
from a mixture of standard E and Z guggulsterones with different
validatory parameters including linearity, selectivity and specificity, accuracy, auto-injector, flow-rate, recoveries, limit of
detection and limit of quantification (as per norms of
International conference of Hormonization). Present findings
revealed the role of environmental factors on biosynthesis of
guggulsterone isomers under natural conditions.
Not Available
 
Date 2018-11-14T11:47:48Z
2018-11-14T11:47:48Z
2015-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/Publication/handle/123456789/11320
 
Language English
 
Relation Not Available;
 
Publisher Not Available