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Assessment of Genetic Diversity in Trigonella foenum-graecum Based on Nuclear Ribosomal DNA, Internal Transcribed Spacer and RAPD Analysis

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Title Assessment of Genetic Diversity in Trigonella foenum-graecum Based on Nuclear Ribosomal DNA, Internal Transcribed Spacer and RAPD Analysis
Not Available
 
Creator R. K. Kakani
S. K. Singh
Anjly Pancholy
R. S. Meena
Rakesh Pathak
Aparna Raturi
 
Subject Trigonella foenum-graecum
Fenugreek
Genetic diversity
RAPD
rDNA diversity
DNA bar coding
 
Description Not Available
Fenugreek (Trigonella foenum-graecum) is
receiving global attention due to rare medicinal properties
of significance to human health. Gene banks possess scanty
germplasm and very little background information regarding
its genetic variability that has hampered its improvement.
We investigated the extent of variability among 17
Indian varieties of fenugreek using phenotypic and genetic
markers. Multilocus genotyping by ten random amplified
polymorphic DNA (RAPD) primers detected an average of
intraspecific variations amounting to 64.7% polymorphism
in banding patterns. Analysis of molecular variance
indicated that a greater proportion of total genetic variation
exists within population (91%) rather than among populations.
Higher values of Nei’s gene diversity (h) and
Shannon Information Index (i) and genetic distance analysis
validate higher genetic diversity among Indian fenugreek
varieties. SNPs at 14 sites of rDNA region revealed further
lineages of distinct varieties with main RAPD clusters. The
representative sequences of each subgroup and all distinct
varieties have been submitted to NCBI database and
assigned Gen Accession numbers HM 176640–176649.
The measures of relative genetic distances among varieties
of fenugreek did not completely correlate with the
geographical distances of places of their development.
The homogeneous phenotypic markers proved insufficient
in exhibiting genetic divergence among fenugreek varieties
studied. Eventually, the knowledge of their genetic relationships,
DNA bar coding and phylogenies might contribute
for the designing of intraspecific crosses between cultivars
of this fenugreek collection with potential interest in seed
spices breeding programme.Not Available
Not Available
 
Date 2019-05-14T11:55:51Z
2019-05-14T11:55:51Z
2011-01-01
 
Type Research Paper
 
Identifier 33
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/19486
 
Language English
 
Relation Not Available;
 
Publisher Springer