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In vitro hardening and acclimatization of seedling of Dendrobium hybrid

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Title In vitro hardening and acclimatization of seedling of Dendrobium hybrid
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Creator Ramgopal Devadas
Jintu Das
Dwijendra Barman
 
Subject Dendrobium hybrid, in vitro hardening
 
Description Not Available
In tissue culture, transferring of small plantlets without proper in vitro hardening results in higher mortality rate. An effort was therefore made using Dendrobium hybrid (HBD-V-19.5-10-SPT) towards devising the ways and means to overcome the mortality problems. The two leaf stage plants were used as explants for the experiment and were cultured in half strength Nitch basal media lacking in sucrose, vitamins and organic component; but having sterilized bricks pieces, charcoal and coconut husk. The explants 1 cm (appx.) in size were sterilized twice before mixing with media @ 25 g each of brick pieces, charcoal and coconut husk used in combination of 1:1 ratio i.e., brick pieces + charcoal, brick pieces + coconut husk, coconut husk _ charcoal and control, with and without activated charcoal (AC). After six months, plantlets were transferred to community pots containing respective potting mixture as that of in in vitro hardeing and plantlets from the control treatment were potted in potting mixture comprising brick + charcoal + coconut husk (1:1:1) and survival % was recorded after three months. The results revealed cent per cent survivability in the treatments of 'brick pieces + charcoal' and 'brick pieces + coconut husk'.
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Date 2019-12-04T08:54:26Z
2019-12-04T08:54:26Z
2008-04-10
 
Type Presentation
 
Identifier Devadas, R.; Das, J.; Barman, D.; 2008. In-viro hardening and acclimatization of seedlings of Dendrobium hybrid. In: National Conference on Orchids: Science and Society at Bangalore, 10-12, April 2008 The Orchid Society of India (Accepted & abstract Pg 138)
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http://krishi.icar.gov.in/jspui/handle/123456789/27222
 
Language English
 
Relation Not Available;
 
Publisher The Orchid Society of India (TOSI)