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Screening of capsicum exotic and indigenous lines for resistance against Pepper mild mottle virus

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Title Screening of capsicum exotic and indigenous lines for resistance against Pepper mild mottle virus
Not Available
 
Creator Nidhi Kumari
Priyankaben Patel
P.N. Sharma
 
Subject Capsicum, polyhouse, PMMoV, resistance, management
 
Description Not Available
Capsicum is an important offseason vegetable crop among the farmers of Himachal Pradesh (H.P.) and widely
grown under protected cultivation. In recent years extensive use of hybrids from many unknown sources under the
polyhouse cultivation has escalated the incidence of many viral diseases including seed-borne viruses like Pepper
mild mottle virus (PMMoV). The use of genetic resistance is the most feasible strategy for plant virus management.
In this study a panel of one hundred and seventy-seven capsicum genotypes including the local landraces, high
yielding recommended cultivars and exotic collections were screened for resistance against PMMoV-HP1 isolate.
The reaction of the genotypes was recorded and validated using Double antibody sandwich-Enzyme linked
immunosorbant assay (DAS-ELISA) and reverse transcriptase-Polymerase chain reaction (RT-PCR). Out of 177
lines, only two accessions PI-159236 and PI-260429 have shown resistance against PMMoV-HP1. All the other
accession lines were susceptible to the disease showing mottling, puckering and mild mosaic on the leaves after 10-
14 days of inoculations. The DAS-ELISA assay using PMMoV specific antiserum exhibited strong reaction with an
OD value at 405 nm between the range of 0.666- 1.564 which was almost 3-4 times more than the negative control
(0.181) in susceptible accessions. In RT-PCR, susceptible accessions yielded an amplification product of ~730 bp,
while no amplification was observed in the case of PI-159236 and PI-260429 inoculated with PMMoV-HP1. These
two lines can be used in breeding programs to develop PMMoV resistant high yielding cultivar
Not Available
 
Date 2022-09-15T05:05:39Z
2022-09-15T05:05:39Z
2020-06-16
 
Type Research Paper
 
Identifier Not Available
2249-8788
http://krishi.icar.gov.in/jspui/handle/123456789/74202
 
Language English
 
Relation Not Available;
 
Publisher Not Available