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Induction and morpho-chemical characterization of Stevia rebaudiana colchiploids

Indian Agricultural Research Journals

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Title Induction and morpho-chemical characterization of Stevia rebaudiana colchiploids
 
Creator YADAV, ASHOK KUMAR
SINGH, S
YADAV, SUBHASH C
DHYANI, D
BHARDWAJ, GARIMA
SHARMA, ANJALI
SINGH, BIKRAM
 
Subject Autotetraploid, Colchiploid, Flow-cytometry, Mixoploid, Scanning Electron Microscopy, Stevia rebaudiana
 
Description A study was conducted to produce higher biomass-yielding stevia Stevia rebaudiana (Bertoni) with larger leaf sizeand high glycoside content. To improve leaf yield and sweet glycoside content found in the leaves of the plant, polyploidywas induced by treating the seeds with colchicine. Treatment of seeds with 0.2% colchicine and above (up to 0.6%)reduced the survival rate of stevia young seedlings to below 50%. Maximum number of polyploids (tetraploids) wereobtained with 0.6% colchicine treatment for 24 hr; mixoploidy was observed at 0.2% colchicine treatment for 6 hr. Ithowever needs further experimentation for more precise information. The nuclear DNA content (2C-value of diploidStevia rebaudiana variety Madhuguna) was estimated by flow-cytometry (FCM) to be 2.72pg. Polyploids C7-3-4, C7-35,C9-1-1 and C9-4-3 could be distinguished from diploid control plants as they had double the DNA content, suggesting autotetraploidy. Chromosome count in root-tip cells of polyploids (2n=44) confirmed their tetraploid status as compared with the control (2n=22). The autotetraploids in stevia had significantly increased leaf size, thickness and chlorophyll content and reduced internode length. Tetraploid stevia genotypes are important germplasm for further improvement of stevia biomass and glycoside content.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2013-03-08
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/27981
 
Source The Indian Journal of Agricultural Sciences; Vol 83, No 2 (2013)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/27981/12689
 
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