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Transboundary introduction of potato-infecting Ralstonia solanacearum in the Andaman Islands revealed by multilocus sequence typing: A potential threat to island agriculture

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Title Transboundary introduction of potato-infecting Ralstonia solanacearum in the Andaman Islands revealed by multilocus sequence typing: A potential threat to island agriculture
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Creator K SAKTHIVEL, A BALA, MASHAJYOTHI, SOOBEDAR YADAV , V BASKARAN , K ABIRAMI4, R K GAUTAM and A KUMAR
 
Subject Potato bacterial wilt; R. solanacearum; host range; Phylotype-I; MLST; Andaman islands
 
Description Not Available
Wilting of potato plants with an incidence of 20–30 % was observed for the first time in the agricultural farms of
Andaman Islands, India. The infected plants showed wilting syndrome that included downward drooping of
leaves, yellowing, and collapse of the entire plants. Characteristic milky-white exudate from the infected stem
indicated bacterial etiology of the disease. Upon streaking onto 2, 3, 5 triphenyl-tetrazolium chloride amended
nutrient medium, the bacterial exudate yielded characteristic creamy-white, fluidal, irregular colonies with the
pink center. Upon inoculation, the randomly picked bacterial colonies, AN_PRSGr and AN_PRSCh, representing the two locations, incited wilt symptoms on one-month-old potato plants. The host range studies
revealed that the isolates were pathogenic on tomato and eggplant but non-pathogenic to chili and Solanum
torvum (wild eggplant). The 16S rRNA gene sequencing and the Ralstonia-specific PCR test confirmed the
identity of AN_PRSGr and AN_PRSCh as Ralstonia solanacearum. Intra-species level classification revealed
their identity as strains of race 1, biovar 3, and phylotype-I. Multilocus sequence typing (MLST)-based in-depth
sequence alignment for phylogenetic analysis revealed the isolates AN_PRSGr and AN_PRSCh clustered with
two mainland race 1/biovar 3/phylotype-I isolates of Kerala, India. However, the allelic profile-based goeBURST-analysis placed them as singletons in the global collection of Ralstonia solanacearum, conforming
intra-racial/ intra-phylotype diversity within race 1/biovar3/phylotype-I strains. The molecular characterization by MLST revealed that the isolates shared several alleles with isolates reported from mainland India suggesting
the transboundary movement of the pathogen, its introduction, and subsequent spread in the island.
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Date 2021-11-25T08:54:20Z
2021-11-25T08:54:20Z
2021-09-01
 
Type Research Paper
 
Identifier K SAKTHIVEL, A BALA, MASHAJYOTHI, SOOBEDAR YADAV , V BASKARAN , K ABIRAMI4, R K GAUTAM and A KUMAR. 2021. Transboundary introduction of potato-infecting Ralstonia solanacearum in the Andaman Islands revealed by multilocus sequence typing: A potential threat to island agriculture. Journal of Biosciences 46(3)
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http://krishi.icar.gov.in/jspui/handle/123456789/67824
 
Language English
 
Relation Not Available;
 
Publisher Indian Academy of Sciences