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De novo genome sequencing of mycoparasite Mycogone perniciosa strain MgR1 sheds new light on its biological complexity.

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Title De novo genome sequencing of mycoparasite Mycogone perniciosa strain MgR1 sheds new light on its biological complexity.
Mycogone perniciosa strain MgR1
 
Creator Anil Kumar, VP Sharma, Satish Kumar and Manoj Nath
 
Subject Genome sequencing · Pathogenic genes · Mycoparasitism · Mycogone perniciosa · Wet Bubble Disease · Agaricus bisporus
 
Description Not Available
Mycogone perniciosa is a mycoparasite causing Wet Bubble Diseases (WBD) of Agaricus bisporus. In the present study, the whole genome of M. perniciosa strain MgR1 was sequenced using Illumina NextSeq500 platform. This sequencing generated 8.03 Gb of high-quality data and a draft genome of 39 Mb was obtained through a de novo assembly of the high-quality reads. The draft genome resulted into prediction of 9276 genes from the 1597 scafolds. NCBI-based homology analysis revealed the identifcation of 8660 genes. Notably, non-redundant protein database analysis of the M. perniciosa strain MgR1 revealed its close relation with the Trichoderma arundinaceum. Moreover, ITS-based phylogenetic analysis showed the highest similarity of M. perniciosa strain MgR1 with Hypomyces perniciosus strain CBS 322.22 and Mycogone perniciosa strain
PPRI 5784. Annotation of the 3917 genes of M. perniciosa strain MgR1 grouped in three major categories viz. biological process (2583 genes), cellular component (2013 genes), and molecular function (2919 genes). UniGene analysis identifed 2967 unique genes in M. perniciosa strain MgR1. In addition, prediction of the secretory and pathogenicity-related genes based on the fungal database indicates that 1512 genes (16% of predicted genes) encode for secretory proteins. Moreover, out of 9276 genes, 1296 genes were identifed as pathogenesis-related proteins matching with 51 fungal and bacterial genera. Overall, the key pathogenic genes such as lysine M protein domain genes, G protein, hydrophobins, and cytochrome P450 were also observed. The draft genome of MgR1 provides an understanding of pathogenesis of WBD in A. bisporus and could
be utilized to develop novel management strategies.
Not Available
 
Date 2022-08-05T07:31:32Z
2022-08-05T07:31:32Z
2021-06-17
 
Type Journal
 
Identifier 1. Anil Kumar, VP Sharma, Satish Kumar and Manoj Nath. 2021. De novo genome sequencing of mycoparasite Mycogone perniciosa strain MgR1 sheds new light on its biological complexity. Brazilian Journal of Microbiology 52:1545–1556 DOI: 10.1007/s42770-021-00535-x
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http://krishi.icar.gov.in/jspui/handle/123456789/73715
 
Language English
 
Relation Not Available;
 
Publisher Springer Nature