Mechanism and kinetics of chlorpyrifos co-metabolism by using environment restoring microbes isolated from rhizosphere of horticultural crops unde3r subtropics
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Title |
Mechanism and kinetics of chlorpyrifos co-metabolism by using environment restoring microbes isolated from rhizosphere of horticultural crops unde3r subtropics
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Creator |
Govind Kumar, Shatrohan Lal, Sumit K Soni, Shailendra K Maurya, Pradeep K Shukla, Parul Chaudhary, A K Bhattacherjee and Neelima Garg
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Subject |
Chlorpyrifos, biodegradation, plant growth promotion, consortium, ERM C-1
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Description |
Not Available
The indiscriminate use of organophosphate insecticide chlorpyrifos in agricultural crops causes significant soil and water pollution and poses a serious threat to the global community. In this study, a microbial consortium ERM C-1 containing bacterial strains Pseudomonas putida T7, Pseudomonas aeruginosa M2, Klebsiella pneumoniae M6, and a fungal strain Aspergillus terreus TF1 was developed for the effective degradation of chlorpyrifos. Results revealed that microbial strains were not only utilizing chlorpyrifos (500 mg L-1) but also coupled with plant growth-promoting characteristics and laccase production. PGP traits, that is, IAA (35.53, 45.53, 25.19, and 25.53 mg mL-1), HCN (19.85, 17.85, 12.18, and 9.85 mg mL-1), and ammonium (14.73, 16.73, 8.05, and 10.87 mg mL-1) production, and potassium (49.53, 66.72, 46.14, and 52.72 mg mL-1), phosphate (52.37, 63.89, 33.33, and 71.89 mg mL-1), and zinc (29.75, 49.75, 49.12, and 57.75 mg mL-1) solubilization tests were positive for microbial strains T7, M2, M6, and TF1, respectively. The laccase activity by ERM C-1 was estimated as 37.53, 57.16, and 87.57 enzyme U mL1 after 5, 10, and 15 days of incubation, respectively. Chlorpyrifos degradation was associated with ERM C-1 and laccase activity, and the degree of enzyme activity was higher in the consortium than in individual strains. The biodegradation study with developed consortium ERM C-1 showed a decreased chlorpyrifos concentration from the 7th day of incubation (65.77% degradation) followed by complete disappearance (100% degradation) after the 30th day of incubation in the MS medium. First-order degradation kinetics with a linear model revealed a high k -day value and low t1/2 value in ERM C-1. The results of HPLC and GC-MS analysis proved that consortium ERM C-1 was capable of completely removing chlorpyrifos by co-metabolism mechanism. Not Available |
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Date |
2022-09-15T05:50:43Z
2022-09-15T05:50:43Z 2022-07-26 |
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Type |
Research Paper
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Identifier |
Kumar G, Lal S, Soni SK, Maurya SK, Shukla PK, Chaudhary P, Bhattacherjee AK and Garg N (2022) Mechanism and kinetics of chlorpyrifos co-metabolism by using environment restoring microbes isolated from rhizosphere of horticultural crops under subtropics. Frontiers in Microbiology 13: 891870. doi: 10.3389/fmicb.2022.891870
doi: 10.3389/fmicb.2022.891870 http://krishi.icar.gov.in/jspui/handle/123456789/74214 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Frontiers in Microbiology
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