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Mechanism and kinetics of chlorpyrifos co-metabolism by using environment restoring microbes isolated from rhizosphere of horticultural crops unde3r subtropics

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Title Mechanism and kinetics of chlorpyrifos co-metabolism by using environment restoring microbes isolated from rhizosphere of horticultural crops unde3r subtropics
Not Available
 
Creator Govind Kumar, Shatrohan Lal, Sumit K Soni, Shailendra K Maurya, Pradeep K Shukla, Parul Chaudhary, A K Bhattacherjee and Neelima Garg
 
Subject Chlorpyrifos, biodegradation, plant growth promotion, consortium, ERM C-1
 
Description Not Available
The indiscriminate use of organophosphate insecticide chlorpyrifos in
agricultural crops causes significant soil and water pollution and poses a
serious threat to the global community. In this study, a microbial consortium
ERM C-1 containing bacterial strains Pseudomonas putida T7, Pseudomonas
aeruginosa M2, Klebsiella pneumoniae M6, and a fungal strain Aspergillus
terreus TF1 was developed for the effective degradation of chlorpyrifos.
Results revealed that microbial strains were not only utilizing chlorpyrifos
(500 mg L-1) but also coupled with plant growth-promoting characteristics
and laccase production. PGP traits, that is, IAA (35.53, 45.53, 25.19, and
25.53 mg mL-1), HCN (19.85, 17.85, 12.18, and 9.85 mg mL-1), and ammonium
(14.73, 16.73, 8.05, and 10.87 mg mL-1) production, and potassium (49.53,
66.72, 46.14, and 52.72 mg mL-1), phosphate (52.37, 63.89, 33.33, and 71.89 mg
mL-1), and zinc (29.75, 49.75, 49.12, and 57.75 mg mL-1) solubilization tests
were positive for microbial strains T7, M2, M6, and TF1, respectively. The
laccase activity by ERM C-1 was estimated as 37.53, 57.16, and 87.57 enzyme
U mL􀀀1 after 5, 10, and 15 days of incubation, respectively. Chlorpyrifos
degradation was associated with ERM C-1 and laccase activity, and the degree
of enzyme activity was higher in the consortium than in individual strains.
The biodegradation study with developed consortium ERM C-1 showed a
decreased chlorpyrifos concentration from the 7th day of incubation (65.77%
degradation) followed by complete disappearance (100% degradation) after
the 30th day of incubation in the MS medium. First-order degradation kinetics
with a linear model revealed a high k -day value and low t1/2 value in ERM C-1.
The results of HPLC and GC-MS analysis proved that consortium ERM C-1 was
capable of completely removing chlorpyrifos by co-metabolism mechanism.
Not Available
 
Date 2022-09-15T05:50:43Z
2022-09-15T05:50:43Z
2022-07-26
 
Type Research Paper
 
Identifier Kumar G, Lal S, Soni SK, Maurya SK, Shukla PK, Chaudhary P, Bhattacherjee AK and Garg N (2022) Mechanism and kinetics of chlorpyrifos co-metabolism by using environment restoring microbes isolated from rhizosphere of horticultural crops under subtropics. Frontiers in Microbiology 13: 891870. doi: 10.3389/fmicb.2022.891870
doi: 10.3389/fmicb.2022.891870
http://krishi.icar.gov.in/jspui/handle/123456789/74214
 
Language English
 
Relation Not Available;
 
Publisher Frontiers in Microbiology