Record Details

Efficient and simple procedure for isolation of RNA from pulp and peel tissues of ripe banana.

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Efficient and simple procedure for isolation of RNA from pulp and peel tissues of ripe banana.
Not Available
 
Creator S Backiyarani, S Uma, MS Saraswathi, A Saravankumar
 
Subject Banana, Pulp peel, RNA isolation
 
Description Not Available
Sensitive techniques of molecular biology, such as identification of differentially expressed
genes, transcriptional profiling etc., require a high quality RNA in suitable quantities. Isolation of good
quality RNA fro1n banana pulp and peel tissues is troublesome and challenging owing to rich phenolic
compounds and polysaccharides that coprecipitate with nucleic acids. Interaction of phenols with nucleic
acids leads to oxidation and degradation of RN A making it unsuitable for downstream processes. We have
developed a protocol to isolate good quality RNA from banana fruit pulp and peel tissues This involves
two precipitation steps with sodium acetate with I 00 per cent ethanol and reducing the precipitation time
which led to the reduction in loss of RNA and risk of degradation. The protocol developed is simple, fast
and can extract 81.85 and 40.54 μgig of pulp and peel tissues, respectively. The absorbance ranged from
I. 9-2.0 at the ratio of260/280 indicating very high quality of RNA suitable for molecular analyses. RNA
purity was confirmed through reverse transcription-polymerase chain reaction (RT-PCR) by using J3-1,3
glucanase primer pair. The clear banding pattern obtained in RT-PCR analysis revealed that RNA isolated
through this protocol could be used for further downstrea1n processes.
ICAR, ICAR-NRCB
 
Date 2020-12-17T09:39:55Z
2020-12-17T09:39:55Z
2013-09-22
 
Type Research Paper
 
Identifier Efficient and simple procedure for isolation of RNA from pulp and peel tissues of ripe banana. 2013.S Backiyarani, S Uma, MS Saraswathi, A Saravankumar Asian Journal of Horticulture 8 (2), 478-481
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/43513
 
Language English
 
Relation Not Available;
 
Publisher HAS