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Micropropagation and total alkaloid extraction of Indian snake root

Indian Agricultural Research Journals

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Title Micropropagation and total alkaloid extraction of Indian snake root
 
Creator BAHUGUNA, RAJEEV NAYAN
JOSHI, ROHIT
SINGH, GIRIRAJ
SHUKLA, ALOK
GUPTA, RAMWANT
BAINS, GURDEEP
 
Subject Alkaloids; Endangered species; Organogenesis; Rauwolfia serpentina; Shoot multiplication
 
Description An efficient and reproducible protocol for clonal propagation was developed for Indian snake root (Rauwolfia serpentina L. Benth. ex. Kurtz.) through indirect and direct organogenesis using nodal segment, shoot apices and leaves as explants. For indirect organogenesis using leaf and nodal segments, most efficient callus induction was obtained on MS + 2.0 mg /litre 2, 4-D + 1.0 mg /litre BAP. Proliferated calli further showed highest regeneration response in MS + 1.0 mg / litre BAP + 0.1 mg /litre KIN + 0.1 mg /litre GA3. However, for direct organogenesis using shoot apices and nodal segments, most efficient regeneration was observed in MS +1.0 mg /litre BAP + 0.1 mg /litre KIN + 0.1 mg /litre GA3. For complete removal of contamination due to endophytic microflora, 6.0 g /litre bavistin and 100.0 mg /litre streptomycin was found most effective. Regenerated plantlets obtained both through direct and indirect organogenesis showed multiple shooting on MS + 1.0 mg /litre BAP + 0.1 mg /litre NAA and excellent rooting in MS + 1.0 mg /litre IBA + 1.0 mg /litre IAA. Well rooted plantlets were successfully acclimatized in greenhouse. Plantlets developed through shoot apices showed maximum height, number of shoots and number of leaves. Quantitative estimation of crude alkaloids from six weeks old regenerated plantlets was found similar to that obtained from leaves and stems of one year old field grown plant. The developed protocol can be used for en masse propagation and alkaloid extraction of Rauwolfia serpentina.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2011-12-07
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/13308
 
Source The Indian Journal of Agricultural Sciences; Vol 81, No 12 (2011)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/13308/6697
 
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