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Rapid protocol for callus induction and differentiation of roots and shoots in Dioscorea alata—a medicinal plant

Indian Agricultural Research Journals

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Title Rapid protocol for callus induction and differentiation of roots and shoots in Dioscorea alata—a medicinal plant
 
Creator KUMAR, ASHWANI
GOYAL, S C
KAJLA, SUBHASH
SHARMA, NEELAM
 
Subject Callus induction, Differentiation, NAA, Yam
 
Description The present study was carried out to investigate the effect of different concentrations of growth regulators on in vitro differentiation of Dioscorea through nodal explants which provide a fast, reliable and alternate method of multiplication and facilitate ex situ conservation of this valuable plant species. Nodal explants excised from in vivo grown plants were cultured on Murashige and Skoog (MS) medium supplemented with different combinations of plant growth substances. The maximum (100 per cent) callus induction as well as growth was obtained on NAA (2.5 mg/l). The induced callus was transferred onto the nutrient media supplemented with different combinations of growth regulators for the differentiation of roots and shoots. Roots were developed on MS medium + NAA (2.0 mg/l) + IBA (0.5 mg/l), shoots on MS + BAP (2.0 mg/l) + NAA (0.5 mg/l) and roots-shoots on MS + NAA (2.5 mg/l) + 2, 4-D (2.0 mg/l) from the callus.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2014-01-28
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/37165
 
Source The Indian Journal of Agricultural Sciences; Vol 84, No 1 (2014)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/37165/16603
 
Rights Copyright (c) 2014 The Indian Journal of Agricultural Sciences