Record Details

DsRNA-mediated protection against two isolates of Papaya ringspot virus through topical application of dsRNA in papaya

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title DsRNA-mediated protection against two isolates of Papaya ringspot virus through topical application of dsRNA in papaya
Not Available
 
Creator T. Vadlamudi, B. L. Patil, A. Kaldis, D. V. Saigopal, R. Mishra, M. Berbati and A. Voloudakis*
 
Subject dsRNA, Papaya ringspot virus
 
Description Not Available
Papaya ringspot virus (PRSV) infections in papaya result in heavy yield losses, severely affecting the papaya industry worldwide, and hence warranting for effective control measures. In the past, transgenic papaya cultivars were developed that overexpressed parts of the PRSV genome and exhibited high levels of virus resistance. In the present study, a non-transgenic approach was employed, in which in vitro produced dsRNA molecules derived from a PRSV isolate from South India (PRSV-Tirupati) was tested for dsRNA-mediated protection against two isolates of PRSV through topical application of the dsRNA on papaya. The results showed that the dsRNA molecules from both the coat protein (CP) and helper component-proteinase (HC-Pro) genes of the PRSV-Tirupati isolate conferred 100 % resistance against PRSV-Tirupati infection. Further, the same dsRNA molecules were highly effective against the PRSV-Delhi isolate on the papaya cv. Pusa Nanha, conferring a resistance of 94 % and 81 %, respectively. Systemic papaya leaves of the dsRNA-treated plants were virus-free at 14 days post-inoculation, confirming the robustness of this non-transgenic virus control strategy. In contrast, the control TMV dsRNA did not protect against the PRSV infection. This study on the topical application of dsRNA opened up a new avenue for the control of papaya ringspot disease worldwide.
Not Available
 
Date 2020-06-04T05:03:50Z
2020-06-04T05:03:50Z
2020-01-01
 
Type Article
 
Identifier T. Vadlamudi, B. L. Patil, A. Kaldis, D. V. Saigopal, R. Mishra, M. Berbati and A. Voloudakis* (2020) DsRNA-mediated protection against two isolates of Papaya ring spot virus through topical application of dsRNA in papaya. Jr. of Virological Methods, 275:113750. doi: 10.1016/j.jviromet.2019.113750
https://doi.org/10.1016/j.jviromet.2019.113750
http://krishi.icar.gov.in/jspui/handle/123456789/36829
 
Language English
 
Relation Not Available;
 
Publisher Elsevier Limited