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Improvement of banana cv. Rasthali (Silk, AAB) against Fusarium oxysporum f.sp. cubense (VCG 0124/5) through induced mutagenesis: Determination of LD50 specific to mutagen, explants, toxins and in vitro and in vivo screening for Fusarium wilt resistance

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Title Improvement of banana cv. Rasthali (Silk, AAB) against Fusarium oxysporum f.sp. cubense (VCG 0124/5) through induced mutagenesis: Determination of LD50 specific to mutagen, explants, toxins and in vitro and in vivo screening for Fusarium wilt resistance
Not Available
 
Creator MS Saraswathi, *, G Kannan , S Uma, R Thangavelu & S Backiyarani
 
Subject Di ethyl sulphate, Ethyl methane sulfonate, Fusaric acid, Fungal resistance, Musa sp., Sodium azide
 
Description Not Available
Shoot tips and in vitro grown proliferating buds of banana cv. Rasthali (Silk, AAB) were treated with various
concentrations and durations of chemical mutagens viz., EMS, NaN3 and DES. LD50 for shoot tips based on 50% reduction in fresh weight was determined as 2% for 3 h, 0.02% for 5 h and 0.15%for 5 h, while for proliferating buds, they were 0.6% for 30 min, 0.01% for 2 h and 0.06% for 2 h for the mutagens EMS, NaN3 and DES, respectively. Subsequently, the mutated explants were screened in vitro against fusarium wilt using selection agents like fusaric acid and culture filtrate. LD50 for in vitro selection agents calculated based on 50% survival of explants was 0.050 mM and 7% for fusaric acid and culture filtrate, respectively and beyond which a rapid decline in growth was observed. This was followed by pot screening which led to the identification of three putative resistant mutants with an internal disease score of 1 (corm completely clean, no vascular discolouration). The putative mutants identified in the present study have also been mass multiplied in vitro.
BRNS
 
Date 2020-12-04T09:30:32Z
2020-12-04T09:30:32Z
2014-12-30
 
Type Research Paper
 
Identifier Saraswathi MS, Kannan G, Uma S, Thangavelu R, Backiyarani S. Improvement of banana cv. Rasthali (Silk, AAB) against Fusarium oxysporum f.sp. cubense (VCG 0124/5) through induced mutagenesis: Determination of LD50 specific to mutagen, explants, toxins and in vitro and in vivo screening for Fusarium wilt resistance. Indian J Exp Biol. 2016;54(5):345-353.
0019-5189
http://krishi.icar.gov.in/jspui/handle/123456789/42964
 
Language English
 
Relation Not Available;
 
Publisher NISCAIR, CSIR