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Cost-effective tissue culture media for large-scale propagation of three commercial banana (Musa spp.) varieties

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Title Cost-effective tissue culture media for large-scale propagation of three commercial banana (Musa spp.) varieties
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Creator MS Saraswathi, S Uma, G Kannan, M Selvasumathi, MM Mustaffa,
 
Subject Banana; tissue culture; propagation; ISSR markers
 
Description Not Available
Cost-effective tissue culture protocols have been established for the commercial multiplication of three banana varieties, ‘Rasthali’ (AAB – Silk), ‘Grand Naine’ (AAA – Cavendish), and
‘Udhayam’ (ABB – Pisang Awak). Reverse osmosis water and 3% (w/v) table sugar were used
as the low-cost water and carbon source, respectively. Six different gelling agent treatments
were tested: sago alone (T1), Isabgol alone (T2), sago + agar (T3), Isabgol + agar (T4), sago +
Isabgol (T5), and agar alone as a control (T6). Full-strength Murashige and Skoog (MS)
medium supplemented with 3 mg l–1 6-benzylaminopurine (BAP) and 1 mg l–1 indole-3-acetic
acid (IAA) were used for culture initiation and subculturing. Rooting was accomplished on
low-cost MS medium containing 1.0 mg l–1 α-napthaleneacetic acid (NAA), 1.0 mg l–1 indole3-butyric acid (IBA), and 250 mg l–1 activated charcoal. Statistical analysis indicated that sago
+ Isabgol (T5) produced the maximum number of shoots (10 per explant) in ‘Udhayam’ and
‘Rasthali’, while sago alone (T1) produced the maximum number of shoots (6 per explant) in
‘Grand Naine’. The genetic stability of tissue-cultured banana plantlets produced using these
low-cost substitutes was assessed using inter-simple sequence repeat (ISSR) markers. The
results indicated that the ISSR profiles of the five treatments and the control (T6) were similar,
indicating genetic stability using these cost-effective tissue culture protocols. Reductions in
cost over the control (l–1 of MS medium) ranged from 65% to 86%, while the per plant
production cost was reduced by 12.5%–20.0%. Adoption of these treatments (T1–T5) as lowcost tissue culture protocols for in vitro propagation would reduce production costs significantly, leading to an expansion of the area planted with tissue-cultured banana, thereby
increasing productivity.
ICAR, ICAR-NRCB
 
Date 2020-12-17T08:56:37Z
2020-12-17T08:56:37Z
2016-02-12
 
Type Research Paper
 
Identifier M.S. Saraswathi, S. Uma, G. Kannan, M. Selvasumathi, M.M. Mustaffa & S. Backiyarani (2016) Cost-effective tissue culture media for large-scale propagation of three commercial banana (Musa spp.) varieties, The Journal of Horticultural Science and Biotechnology, 91:1, 23-29, DOI: 10.1080/14620316.2015.1117227
https://doi.org/10.1080/14620316.2015.1117227
http://krishi.icar.gov.in/jspui/handle/123456789/43508
 
Language English
 
Relation Not Available;
 
Publisher Taylor and Francis