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Modified and simple regeneration pathway from male flower bud of banana (cv. Nedu Nendran AAB) via somatic embryogenesis

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Title Modified and simple regeneration pathway from male flower bud of banana (cv. Nedu Nendran AAB) via somatic embryogenesis
Not Available
 
Creator Uma S.*, Akbar A., Saraswathi M. S., Udhayaanjali K.
 
Subject Musa, banana, plantain, Nendran, embryogenic cell suspension, somatic embryos, cell density
 
Description Not Available
This study was aimed at improving the regeneration capacity of recalcitrant plantain cv. Nedu Nendran (AAB) through modification of culture technique and media composition. Liquid pretreated floral explants exhibited better results in terms of earlier (3–4 months) and higher embryogenic response (4.5%) on solid callus induction media. Embryogenic clusters developed between 4–7 months of culture were transferred to suspension medium. Gradual scale up of medium from 4 to 15 ml in 60 days was found beneficial for better uniformity of cell suspension. Regeneration of somatic embryos on picloram (4.1 μM) supplemented medium exhibited better germination irrespective of the age of embryos. Earlier germination response (by 30 days) and persistence of germination capacity until 120 days was recorded from embryos regenerated in modified regeneration medium. This resulted in a time gain of 30 days and prolonged availability of good explants (somatic embryos) for 30 days over the standard protocol. Minor modifications in the culture techniques and media composition improved the embryogenic capacity and regeneration ability in otherwise a recalcitrant cv. Nedu Nendran.
ICAR, ICAR-NRCB
 
Date 2020-12-17T09:36:55Z
2020-12-17T09:36:55Z
2013-12-04
 
Type Research Paper
 
Identifier Uma S.*, Akbar A., Saraswathi M. S., Udhayaanjali K..2013. Modified and simple regeneration pathway from male flower bud of banana (cv. Nedu Nendran AAB) via somatic embryogenesis. nternational Journal of Innovative Horticulture. 2(1 ): 52- 58.
2320-0286
http://krishi.icar.gov.in/jspui/handle/123456789/43512
 
Language English
 
Relation Not Available;
 
Publisher Indian Journals