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Embryo rescue and plant regeneration in banana (Musa spp.)

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Title Embryo rescue and plant regeneration in banana (Musa spp.)
Not Available
 
Creator S. Uma • S. Lakshmi • M. S. Saraswathi • A. Akbar • M. M. Mustaffa
 
Subject Banana Embryo maturity In vitro culture Zygotic embryo Callus Regeneration
 
Description Not Available
An efficient regeneration protocol for zygotic embryos at varying maturity stages was developed for wild banana (Pisang Jajee (AA)). Embryo ontogeny was studied to determine the best maturity stage for embryo rescue, suitable media and culture conditions (light and dark) for germination and regeneration. The conversion of endosperm from transparent fluid into a semi-solid state was followed by visible embryo development, which commenced only after 70% embryo maturity. Zygotic embryos of Pisang Jajee at different maturity levels were excised and cultured on medium fortified with different concentrations of 6-benzyl adenine (BA) and indole acetic acid (IAA). Zygotic embryos produced callus or plantlets 25 days after initiation. The frequency of callus induction was greater in immature embryos irrespective of the media composition and decreased with increasing maturity. Fully matured embryos regenerated directly into plantlets without producing callus. Immature embryos required medium supplemented with plant growth regulators (PGRs) for successful regeneration. Although the culture conditions had no influence, dark conditions favoured callus induction and plant regeneration.
ICAR, ICAR-NRCB
 
Date 2021-07-19T09:00:10Z
2021-07-19T09:00:10Z
2010-10-17
 
Type Research Paper
 
Identifier Uma, S., Lakshmi, S., Saraswathi, M.S. et al. Embryo rescue and plant regeneration in banana (Musa spp.). Plant Cell Tiss Organ Cult 105, 105–111 (2011). https://doi.org/10.1007/s11240-010-9847-9
https://doi.org/10.1007/s11240-010-9847-9
http://krishi.icar.gov.in/jspui/handle/123456789/48780
 
Language English
 
Relation Not Available;
 
Publisher Springer