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Suitability of stationary phase for LC analysis of biomolecules

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Title Suitability of stationary phase for LC analysis of biomolecules
Not Available
 
Creator Saha S., Walia S., Sharma K., Banerjee Kaushik
 
Subject HPLC stationary phase
Analysis of biomolecules
Carotenoids, tocols, sugars, sterols, curcuminoids
 
Description Not Available
Biologically active compounds such as carotenoids/isoprenoids, vitamins, steroids, saponins, sugars, long chain fatty acids, and amino acids play a very important role in coordinating functions in living organisms. Determination of those substances is indispensable in advanced biological sciences. Engineered stationary phase in LC for the analysis of biomolecules has become easier with the development of chromatographic science. In general, C18 column is being used for routine analysis but specific columns are being used for specific molecule. Monolithic columns are found to have higher efficiency than normal column. Among recent introduction, triacontyl stationary phases, designed for the separation of carotenoid isomers, are widely used for the estimation of carotenoids. In comparison to conventional C18 phases, C30 phases exhibited superior shape selectivity for the separation of isomers of carotenoids. It is also found useful for better elution and analysis of tocopherols, vitamin K, sterols, and fatty acids. Vitamin K, E, and their isomers are also successfully resoluted and analyzed by using C30 column. Amino bonded phase column is specifically used for better elution of sugars, whereas phenyl columns are suitable for the separation and analysis of curcuminoids and taxol. Like triacontyl stationary phase, pentafluorophenyl columns are also used for the separation and analysis of carotenoids. Similarly, HILIC column are best suited for sugar analysis. All the stationary phases are made possible to resolute and analyze the target biomolecules better, which are the future of liquid chromatography. The present article focuses on the differential interaction between stationary phase and target biomolecules. The applicability of these stationary phases are reported in different matrices.
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Date 2020-07-31T06:19:21Z
2020-07-31T06:19:21Z
2019-10-17
 
Type Journal
 
Identifier Saha S., Walia S., Sharma K., Banerjee Kaushik (2019). Suitability of stationary phase for LC analysis of biomolecules. Critical Reviews in Food Science and Nutrition DOI: https://doi.org/10.1080/10408398.2019.1665494
https://doi.org/10.1080/10408398.2019.1665494
http://krishi.icar.gov.in/jspui/handle/123456789/38642
 
Language English
 
Relation Not Available;
 
Publisher Taylor & Francis