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High-sensitivity direct analysis of aflatoxins in peanuts and cereal matrices by ultra-performance liquid chromatography with fluorescence detection involving a large volume flow cell

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Title High-sensitivity direct analysis of aflatoxins in peanuts and cereal matrices by ultra-performance liquid chromatography with fluorescence detection involving a large volume flow cell
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Creator Oulkar D, Goon A., Dhanshetty M., Khan Z., Satav S., Banerjee Kaushik
 
Subject Aflatoxin analysis
Aflatoxins in peanuts and cereals
UPLC-fluorescence detection
 
Description Not Available
This paper reports a sensitive and cost effective method of analysis for aflatoxins B1, B2, G1 and G2. The sample preparation method was primarily optimised in peanuts, followed by its validation in a range of peanut-processed products and cereal (rice, corn, millets) matrices. Peanut slurry [12.5 g peanut + 12.5 mL water] was extracted with methanol: water (8:2, 100 mL), cleaned through an immunoaffinity column and thereafter measured directly by ultra-performance liquid chromatography-fluorescence (UPLC-FLD) detection, within a chromatographic runtime of 5 minutes. The use of a large volume flow cell in the FLD nullified the requirement of any post-column derivatisation and provided the lowest ever reported limits of quantification of 0.025 for B1 and G1 and 0.01 μg/kg for B2 and G2. The single laboratory validation of the method provided acceptable selectivity, linearity, recovery and precision for reliable quantifications in all the test matrices as well as demonstrated compliance with the EC 401/2006 guidelines for analytical quality control of aflatoxins in foodstuffs.
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Date 2020-07-31T06:29:59Z
2020-07-31T06:29:59Z
2017-12-26
 
Type Journal
 
Identifier Oulkar D, Goon A., Dhanshetty M., Khan Z., Satav S., Banerjee Kaushik (2017). High-sensitivity direct analysis of aflatoxins in peanuts and cereal matrices by ultra-performance liquid chromatography with fluorescence detection involving a large volume flow cell. Journal of Environmental Science and Health, Part B 53 (4): 255-260
https://doi.org/10.1080/03601234.2017.1410416
http://krishi.icar.gov.in/jspui/handle/123456789/38656
 
Language English
 
Relation Not Available;
 
Publisher Taylor & Francis