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Simultaneous Identification and Quantification of Three Biologically Active Xanthones in Garcinia Species Using a Rapid UHPLC-PDA Method

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Title Simultaneous Identification and Quantification of Three Biologically Active Xanthones in Garcinia Species Using a Rapid UHPLC-PDA Method
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Creator Azazahemad A. Kureshi
Chirag Dholakiya
Tabaruk Hussain
Amit Mirgal
Siddhesh P. Salvi
Pritam C. Barua
Madhumita Talukdar
C. Beena
Ashish Kar
T. John Zachariah
Premlata Kumari
Tushar Dhanani
Raghuraj Singh
Satyanshu Kumar
 
Subject α-mangostin, β-mangostin, γ-mangostin, Garcinia
 
Description Not Available
Xanthones are well recognized as chemotaxonomic markers for the plants belonging to the genus Garcinia. Xanthones
have many interesting pharmacological properties. Efficient extraction and rapid liquid chromatography
methods are essentially required for qualitative and quantitative determination of xanthones in their natural sources.
In the present investigation, fruit rinds extracts of 8 Garcinia species from India, were prepared with solvents of
varying polarity. Identification and quantification of 3 xanthones, namely, α-mangostin, β-mangostin, and γ-mangostin
in these extracts were carried out using a rapid and validated ultra-high-performance liquid chromatography–
photodiode array detection (UHPLC–PDA) method at 254 nm. γ-Mangostin (3.97 ± 0.05 min) was first eluted, and
it was followed by α-mangostin (4.68 ± 0.03 min) and β-mangostin (5.60 ± 0.04 min). The calibration curve for
α-mangostin, β-mangostin, and γ- mangostin was linear in the concentration range 0.781–100 μg/mL. α-Mangostin
was quantified in all 4 extracts of Garcinia mangostana. Its content (%) in hexane, chloroform, ethyl acetate,
and methanol extracts of G. mangostana was 10.36 ± 0.10, 4.88 ± 0.01, 3.98 ± 0.004, and 0.044 ± 0.002, respectively.
However, the content of α-mangostin was below the limit of detection or limit of quantification in the extracts
of other Garcinia species. Similarly, β-mangostin was quantified only in hexane (1.17 ± 0.01%), chloroform
(0.39 ± 0.07%), and ethyl acetate (0.28 ± 0.03%) extracts of G. mangostana. γ-Mangostin was quantified in all 4
extracts of G. mangostana. Its content (%) in hexane, chloroform, ethyl acetate, and methanol extracts of G. mangostana
was 0.84 ± 0.01, 1.04 ± 0.01, 0.63 ± 0.04, and 0.15 ± 0.01, respectively. γ-Mangostin was also quantified
in hexane (0.09 ± 0.01), chloroform (0.05 ± 0.01), and ethyl acetate (0.03 ± 0.01) extracts of G. cowa, ethyl acetate
extract of G. cambogia (0.02 ± 0.01), G. indica (0.03 ± 0.01), and G. loniceroides (0.07 ± 0.01). Similarly, γ-mangostin
was quantified in 3 extracts of G. morella, namely, hexane (0.03 ± 0.01), chloroform (0.04 ± 0.01), and
methanol (0.03 ± 0.01). In the case of G. xanthochymus, γ-mangostin was quantified in chloroform (0.03 ± 0.001)
extract only. α-Mangostin and β-mangostin were not detected in any of 4 extracts of G. pedunculata.
Not Available
 
Date 2020-07-31T05:32:25Z
2020-07-31T05:32:25Z
2019-08-22
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/38628
 
Language English
 
Relation Not Available;
 
Publisher Not Available