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Unraveling the mechanisms of resistance to Sclerotium rolfsii in peanut (Arachis hypogaea L.) using comparative RNA-Seq analysis of resistant and susceptible genotypes

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Title Unraveling the mechanisms of resistance to Sclerotium rolfsii in peanut (Arachis hypogaea L.) using comparative RNA-Seq analysis of resistant and susceptible genotypes
Not Available
 
Creator Bosamia T C
Dodia S M
Mishra G P
Ahmad S
Joshi B
Thirumalaisamy P P
Kumar N
Rathnakumar A L
Sangh C
Kumar A
Radhakrishnan T
 
Subject Sclerotium
peanut
transcriptome
 
Description Not Available
Stem rot, a devastating fungal disease of peanut, is caused by Sclerotium rolfsii. RNA-sequencing approaches have been used to unravel the mechanisms of resistance to stem rot in peanut over the course of fungal infection in resistant (NRCG-CS85) and susceptible (TG37A) genotypes under control conditions and during the course of infection. Out of about 290 million reads, nearly 251 million (92.22%) high-quality reads were obtained and aligned to the Arachis duranensis and Arachis ipaensis genomes with the average mapping of 78.91% and 78.61%, respectively. In total, about 48.6% of genes were commonly regulated, while approximately 21.8% and 29.6% of uniquely regulated genes from A. duranensis and A. ipaensis genomes, respectively, were identified. Several annotated transcripts, such as receptor-like kinases, jasmonic acid pathway enzymes, and transcription factors (TFs), including WRKY, Zinc finger protein, and C2-H2 zinc finger, showed higher expression in resistant genotypes upon infection. These transcripts have a known role in channelizing the downstream of pathogen perception. The higher expression of WRKY transcripts might have induced the systemic acquired resistance (SAR) by the activation of the jasmonic acid defense signaling pathway. Furthermore, a set of 30 transcripts involved in the defense mechanisms were validated with quantitative real-time PCR. This study suggested PAMP-triggered immunity as a probable mechanism of resistance, while the jasmonic acid signaling pathway was identified as a possible defense mechanism in peanut. The information generated is of immense importance in developing more effective ways to combat the stem rot disease in peanut.
Not Available
 
Date 2020-08-15T05:32:38Z
2020-08-15T05:32:38Z
2020-08-03
 
Type Research Paper
 
Identifier Bosamia TC, Dodia SM, Mishra GP, Ahmad S, Joshi B, Thirumalaisamy PP, et al. (2020) Unraveling the mechanisms of resistance to Sclerotium rolfsii in peanut (Arachis hypogaea L.) using comparative RNA-Seq analysis of resistant and susceptible genotypes. PLoS ONE 15(8): e0236823. https://doi.org/10.1371/journal.pone.0236823
1932-6203
https://dx.plos.org/10.1371/journal.pone.0236823
http://krishi.icar.gov.in/jspui/handle/123456789/39589
 
Language English
 
Relation Not Available;
 
Publisher PLOS