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Validation of SCAR markers, diversity analysis of male sterile (S-) cytoplasms and isolation of an alloplasmic S-cytoplasm in Capsicum

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Title Validation of SCAR markers, diversity analysis of male sterile (S-) cytoplasms and isolation of an alloplasmic S-cytoplasm in Capsicum
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Creator Rajesh Kumar, Sanjay Kumar, Neeraj Dwivedi, Sanjeet Kumar, Ashutosh Rai, Major Singh, Dasrath Singh Yadav, Mathura Rai
 
Subject Alloplasmic line Capsicum CMS Inter-specific hybrid ORF Pepper SCAR
 
Description Not Available
Cytoplasmic male sterility (CMS) is increasingly being utilized for hybrid seed production of hot pepper
(Capsicum annuum). Two CMS specific sequenced characterized amplified regions (SCARs), viz., atp6607
and coxII708 developed elsewherewere validated in an array of genotypes possessing male sterile (S-) and
normal/male fertile (N-) cytoplasms and their feasible uses in CMS based pepper hybrid breeding have
been elaborated. A set of eightmaintainer and restorer inbreds were crossed on fourCMS lines possessing
two independently isolated and commercially utilized S-cytoplasms (Peterson’s and Reddy’s). Based on
fertility restoration/maintenance reaction of 32 resulted F1s and on the presence of two SCARs (atp6607
and coxII708) in both the S-cytoplasms, it has been concluded that although two S-cytoplasms were
isolated and commercially utilized independently, they are genetically same or similar. Through interspecific
hybridization between C. chacoense and C. annuum, a new alloplasmic S-cytoplasm in the genus
Capsicum has been isolated and the CMS F1 has been advanced to CMS BC1F1.
ICAR
 
Date 2020-09-10T09:17:15Z
2020-09-10T09:17:15Z
2009-02-05
 
Type Research Paper
 
Identifier Kumar Rajesh, Kumar Sanjay, Dwivedi Neeraj, Kumar Sanjeet, Rai Ashutosh, Singh M, Yadav DS and Rai Mathura (2009). Validation of SCAR markers, diversity analysis of male sterile (S-) cytoplasms and isolation of an alloplasmic S-cytoplasm in Capsicum. Scientia Horticulturae 120: 167-172
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http://krishi.icar.gov.in/jspui/handle/123456789/41141
 
Language English
 
Relation Not Available;
 
Publisher Elsevier