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Accumulation of Three Important Bioactive Compounds in Different Plant Parts of Withania somnifera and its Determination by the LC–ESI-MS-MS (MRM) Method

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Title Accumulation of Three Important Bioactive Compounds in Different Plant Parts of Withania somnifera and its Determination by the LC–ESI-MS-MS (MRM) Method
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Creator Narendra A Gajbhiye
Jayanti Makasana
Satyanshu Kumar
 
Subject LC-ESI-MS/MS · Multiple reaction monitoring · Method validation ·withanolides· Withania somnifera
 
Description Not Available
A comprehensive experiment was conducted to study the accumulation pattern and determination of three important bioactive compounds namely withaferin-A (WA), 12-deoxywithastramonolide (WO) and withanolide-A (WD) and its determination by the liquid chromatography/electrospray ionization tandem mass spectrometry (LC–ESI-MS-MS) method in root, stem, fruits and leaves of Withania somnifera. A rapid and sensitive LC–ESI-MS-MS method was developed and validated for the determination of these three important bioactive compounds, having same molecular weight. The multiplereactionmonitoringmethodwasestablishedbytwotransitionsforeachanalyteandintense transitionusedfor quantification. Separation ofthethree analyteswas achievedwithin aruntimeof 5 min on an RP-18 column using a mobile phase consisting of acetonitrile and 0.1% acetic acid in water in an isocratic condition. The developed method was validated as per the ICH guidelines. The developed method was found to be suitable for identification and quantification of WA, WO andWD indifferent plantparts suchas roots, stems, fruitsand leaves ofW.somnifera. Theaccumulation of WAwas highest in leaves samples (8.84±0.37 mg/g) and it was 2.23, 5.85 and 27.26 times higherthanitsconcentrationinfruits,stemsandroots,respectively.WOandWDcontentswerehighest (0.44±0.016 and 0.72±0.016 mg/g, respectively) in root.
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Date 2020-09-05T08:50:17Z
2020-09-05T08:50:17Z
2015-07-06
 
Type Research Paper
 
Identifier Gajbhiye, N. A., Makasana, J., Kumar, S.; Accumulation of Three Important Bioactive Compounds in Different Plant Parts of Withania somnifera and its Determination by the LC–ESI-MS-MS (MRM) Method; Journal of chromatographic science,(2015);53:1749-1756
0021-9665
http://krishi.icar.gov.in/jspui/handle/123456789/41011
 
Language English
 
Relation Not Available;
 
Publisher Oxford University Press