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SSR marker based differentiation of zygotic and nucellar seedlings in mango (Mangifera indica)

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Title SSR marker based differentiation of zygotic and nucellar seedlings in mango (Mangifera indica)
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Creator KAMLESH KUMAR, MANISH SRIVASTAV, SANJAY KUMAR SINGH and ANKIT SINGH
 
Subject Mango, Nucellar, Polyembryony, SSR markers, Zygotic
 
Description Not Available
Non-availability of standard clonal rootstocks in mango (Mangifera indica L.) is considered as an important
hurdle, which has significant impact on orchard productivity. To obtain grafted mango plants, it is important to use
polyembryonic rootstocks since they produce a zygotic and several nucellar plantlets from their seed, which are trueto-
the type and also uniform. It is therefore imperative to device some reliable approach to ascertain the nucellar origin
of seedlings to be used as rootstocks and thus culling out the variable zygotic seedlings in a polyembryonic genotypes
for their use as uniform clonal rootstock. Differentiation of zygotic and nucellar seedlings using SSR markers in mango
rootstock genotypes was undertaken during 2012-2015. Of the 42 SSRs used, 9 primer pairs (LMMA1, LMMA2,
LMMA8, LMMA15, ESTD1, ESTD2, ESTD6, ESTD9 and ESTD10) were found to be informative, while 33 primer
pairs were monomorphic. These nine primer pairs were used for differentiating zygotic and nucellar seedlings. In
Olour rootstock, LMMA1, LMMA2, LMMA8, ESTD6 and ESTD10 primer pairs were informative and ascertained
the zygotic and nucellar origin of seedlings. In Kurukkan rootstock, ESTD1, ESTD2, ESTD6 and ESTD9 primer
pairs differentiated zygotic from nucellar seedlings. In 13-1 rootstock, LMMA8, LMMA15 and ESTD9 discriminated
nucellars from zygotic seedlings. It is concluded that SSR markers were useful in differentiating the zygotic and
nucellar seedlings in polyembryonic mango rootstocks and can be used in combinations to ascertain the origin of
seedlings in polyembryonic mango rootstocks.
Not Available
 
Date 2021-01-21T04:12:32Z
2021-01-21T04:12:32Z
2020-11-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/44911
 
Language English
 
Relation Not Available;
 
Publisher Not Available