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De Novo Assembly of Bitter Gourd Transcriptomes: Gene Expression and Sequence Variations in Gynoecious and Monoecious Lines

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Title De Novo Assembly of Bitter Gourd Transcriptomes: Gene Expression and Sequence Variations in Gynoecious and Monoecious Lines
De Novo Transcriptomes Assembly of Bitter Gourd
 
Creator Anjali Shukla1, V. K. Singh2, D. R. Bharadwaj1, Rajesh Kumar1, Ashutosh Rai1, A. K. Rai3, Raja Mugasimangalam4, Sriram Parameswaran4, Major Singh1*, P. S. Naik1
 
Subject Bitter Gourd, Transcriptomes, Gene Expression
 
Description Not Available
Bitter gourd (Momordica charantia L.) is a nutritious vegetable crop of Asian origin, used as
a medicinal herb in Indian and Chinese traditional medicine. Molecular breeding in bitter
gourd is in its infancy, due to limited molecular resources, particularly on functional markers
for traits such as gynoecy. We performed de novo transcriptome sequencing of bitter gourd
using Illumina next-generation sequencer, from root, flower buds, stem and leaf samples of
gynoecious line (Gy323) and a monoecious line (DRAR1). A total of 65,540 transcripts for
Gy323 and 61,490 for DRAR1 were obtained. Comparisons revealed SNP and SSR variations
between these lines and, identification of gene classes. Based on available transcripts
we identified 80 WRKY transcription factors, several reported in responses to biotic and abiotic
stresses; 56 ARF genes which play a pivotal role in auxin-regulated gene expression
and development. The data presented will be useful in both functions studies and breeding
programs in bitter gourd.
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Date 2021-07-12T08:18:55Z
2021-07-12T08:18:55Z
2015-06-05
 
Type Research Paper
 
Identifier Shukla A, Singh VK, Bharadwaj DR, Kumar R, Rai A, Rai AK, et al. (2015) De Novo Assembly of Bitter Gourd Transcriptomes: Gene Expression and Sequence Variations in Gynoecious and Monoecious Lines. PLoS ONE 10(6): e0128331. doi:10.1371/ journal.pone.0128331
1932-6203
http://krishi.icar.gov.in/jspui/handle/123456789/47733
 
Language English
 
Relation Not Available;
 
Publisher Public Library of Science