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IN VITRO STUDIES ON DIFFERENT MICROBIAL CONSORTIA FOR UTILIZATION OF CHLORMEQUAT CHLORIDE (CCC) AS FOOD SOURCE

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Title IN VITRO STUDIES ON DIFFERENT MICROBIAL CONSORTIA FOR UTILIZATION OF CHLORMEQUAT CHLORIDE (CCC) AS FOOD SOURCE
 
Creator S.D. Ramtake,Kulkarni R.P., Ghosh J.S,
 
Subject Bioremediation,microbial consortia,judicial,stabilized,LCMS-MS
 
Description Not Available
Chlormequat Chloride (CCC) is a very stable compound used as PGR during foundation and forward pruning as growth retardant in vineyards. Because of non judicial use of CCC exceed the current MRLs at European level in the table grapes and India due to human health risks. So there is need for bioremediation to degrade CCC. Utilization abilities of three bacterial species consortia and three fungal species consortia to mineralize CCC at different time intervals were evaluated in this study developed from isolated pure microbes * BAA - Azotobacter indicus and megaterium and Pseudomonas fluorescens, * * *BPB Pseudomonas putida and Bacillus subtilis. Three fungal species consortia * FAA - Aspergillus nigar and Aspergillus awamorii, * * FTT - Trichoderma viridi and Trichoderma harzianum, viridi and Trichoderma harzianum and Aspergillus nigar and Aspergillus awamori and Penicillium notatum were mass multiplied, stabilized in liquid formulation microbial consortiums were screened for their capability of utilizing CCC as sole carbon energy source, and found it was rapidly utilized CCC beyond (500 mg) abundance growth in a PDA and Nutrient Agar medium. The concentration of the CCC in the medium decreased exponentially with the exposure time. It is evident from the data that the maximum growth of bacterial consortium * * BPB was observed in nutrient broth enriched with 2500 mg - 1L and fungal consortium * * * FDC in potato dextrose broth enriched with 1500 mg - 1L CCC with residue 25.14 mg reflecting 99.07 and 98.36 % utilization of CCC respectively, as a food source on LCMS MS. Change in optical density, and spore biomass produced supported the biological transformation further resulting in mineralization of CCC.
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Date 2021-07-08T08:07:29Z
2021-07-08T08:07:29Z
2019-01-28
 
Type Article
 
Identifier Kulkarni R.P.,S.D. Ramtake, Ghosh J.S,(2019)IN VITRO STUDIES ON DIFFERENT MICROBIAL CONSORTIA FOR UTILIZATION OF CHLORMEQUAT CHLORIDE (CCC) AS FOOD SOURCE. International Journal of Current Advanced Research.Vol 8 (1), Pp:17031-17035
2319-6475
http://krishi.icar.gov.in/jspui/handle/123456789/47562
 
Language English
 
Relation Not Available;
 
Publisher Not Available