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Characterization of STP4 promoter in Indian mustard Brassica juncea for use as an aphid responsive promoter

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Title Characterization of STP4 promoter in Indian mustard Brassica juncea for use as an aphid responsive promoter
Not Available
 
Creator Chet Ram
Murali Krishna Koramutla
Rekha Kansal
Ajay Arora
Pradeep K. Jain
Ramcharan Bhattacharya
 
Subject Aphid-responsive genes
Plant inducible promoter
Promoter analysis
Brassica juncea
Agroinfiltration
Transgenics
 
Description Not Available
Objective Brassica juncea, a major oilseed crop,
suffers substantial yield losses due to infestation by
mustard aphids (Lipaphis erysimi). Unavailability of
resistance genes within the accessible gene pool
underpins significance of the transgenic strategy in
developing aphid resistance. In this study, we aimed
for the identification of an aphid-responsive promoter
from B. juncea, based on the available genomic
resources.
Results A monosaccharide transporter gene, STP4 in
B. juncea was activated by aphids and sustained
increased expression as the aphids colonized the
plants. We cloned the upstream intergenic region of
STP4 and validated its stand-alone aphid-responsive
promoter activity. Further, deletion analysis identified
the putative cis-elements important for the aphid
responsive promoter activity.
Conclusion The identified STP4 promoter can
potentially be used for driving high level aphidinducible
expression of transgenes in plants. Use of
aphid-responsive promoter instead of constitutive
promoters can potentially reduce the metabolic burden
of transgene-expression on the host plant.
Not Available
 
Date 2021-07-09T06:01:25Z
2021-07-09T06:01:25Z
2020-07-16
 
Type Research Paper
 
Identifier Not Available
0141-5492
http://krishi.icar.gov.in/jspui/handle/123456789/47576
 
Language English
 
Relation Not Available;
 
Publisher Springer