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Next-generation sequencing reveals endosymbiont variability in cassava whitefly, Bemisia tabaci, across the agro-ecological zones of Kerala, India

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Title Next-generation sequencing reveals endosymbiont variability in cassava whitefly, Bemisia tabaci, across the agro-ecological zones of Kerala, India
Not Available
 
Creator Not Available
ER Harish, ManiChellappan, T MakeshKumar, Deepu Mathew, MT Ranjith, D Girija
 
Subject Arsenophonus, cassava mosaic, NGS, 16S rDNA, symbiotic bacteria
 
Description Not Available
Silverleaf whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is one of the most notorious invasive
insect pests, infesting more than 900 species of plants and spreading more than 200 viral diseases. This polyphagous
agricultural pest harbours diverse bacterial communities in its gut, which perform multiple functions in whiteflies,
including nutrient provisioning, amino acid biosynthesis, and virus transmission. The present exploratory study
compares the bacterial communities associated with silverleaf whitefly infesting cassava, also known as cassava
whitefly, collected from two different zones (zone P: plains; zone H: high ranges), from Kerala, India, using nextgeneration
sequencing of 16S rDNA. The data sets for these two regions consisted of 1 321 906 and 690 661 high-quality
paired-end sequences with mean length of 150 bp. Highly diverse bacterial communities were present in the sample,
containing approximately 3513 operational taxonomic units (OTUs). Sequence analysis showed a marked difference
in the relative abundance of bacteria in the populations. A total of 16 bacterial phyla, 27 classes, 56 orders, 91 families,
236 genera, and 409 species were identified from the P population, against 16, 31, 60, 88, 225, and 355, respectively, in
the H population. Arsenophonus sp. (Enterobacteriaceae), which is important for virus transmission by whiteflies, was
relatively abundant in the P population, whereas in the H population Bacillus sp. was the most dominant group. The
association of whitefly biotypes and secondary symbionts suggests a possible contribution of these bacteria to host
characteristics such as virus transmission, host range, insecticide resistance, and speciation.
ICAR
 
Date 2021-07-19T09:00:58Z
2021-07-19T09:00:58Z
2019-07-08
 
Type Journal
 
Identifier Not Available
https://doi.org/10.1139/gen-2018-0050
http://krishi.icar.gov.in/jspui/handle/123456789/48783
 
Language English
 
Relation Not Available;
 
Publisher Canadian Science Publishing