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[PDF] from isrc.in Endosymbiotic Bacteria Associated with Aphis gossypii Glover (Hemiptera: Aphidae) infesting taro

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Title [PDF] from isrc.in Endosymbiotic Bacteria Associated with Aphis gossypii Glover (Hemiptera: Aphidae) infesting taro
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Creator BG Sangeetha, EV Sreejitha, CA Jayaprakas, ER Harish
 
Subject Endosymbiotic bacteria, aphids, 16S rDNA, molecular characterization, Bacillus
 
Description Not Available
Aphids are one of the important sucking pests of many agriculturally important crops, associated with
a wide variety of bacterial endosymbionts that confer many ecologically relevant traits to the host
insect. Endosymbiotic bacteria (ESB) play a vital role even in the physiology of the host, hence
identification of ESB associated with the aphids will help to develop important strategies for the
management of this noxious pest. In the present study molecular characterization of ESB associated
with the aphid, Aphis gossypii infesting taro was done. Morphological characters of the four strains
revealed that each isolate has different colony characters. Further, the genomic DNA was isolated
from each of the EPB isolates and PCR amplification of 16S rRNA gene was carried out using universal
primers. The 16S rDNA gene sequences of endosymbiotic bacterial isolates were generated by
sequencing the PCR product and were aligned with each other by using BioEDIT software. The nucleotide
sequences were compared with those in the NCBI databases using the Basic Local Alignment Search
and four genus viz., Bacillus, Pesudomonas, Pantoea and Staphylococcus were confirmed. From the
aligned sequences phylogenetic tree was constructed by the Neighbor-Joining method using MEGA
version X.
ICAR
 
Date 2021-07-19T09:01:45Z
2021-07-19T09:01:45Z
2019-12-31
 
Type Journal
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/48786
 
Language English
 
Relation Not Available;
 
Publisher Indian Society for Root Crops