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Assessment of genetic relatedness among Indian mustard (Brassica juncea) genotypes using morphological traits and DNA marker

Indian Agricultural Research Journals

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Title Assessment of genetic relatedness among Indian mustard (Brassica juncea) genotypes using morphological traits and DNA marker
 
Creator Gupta, Ramwant
Chandrashekhar, U S
Yadav, J B
Chakrabarty, S K
Dadlani, M
 
Subject Brassica; DNA; Polymorphism; PIC; PCA; RAPD
 
Description The genetic diversity and relatedness of 17 Indian mustard (Brassica juncea (L.) Czern & Coss) genotypes were assessed with morpho-physiological characteristics and 10 RAPD markers. Molecular parameters, viz. total number of bands, average polymorphic band, average per cent polymorphism, average polymorphic information content (PIC), average expected gene diversity (Hi), Jaccard’s similarity coefficient, principal coordinate analysis (PCA) and dendrogram generated using RAPD markers. Ten RAPD primers generated 62 loci with 50 polymorphic and 12 monomorphic showing 6.2 loci per primer. Primer OPB-01 showed maximum polymorphism (100 %) with maximum PIC (0.95) and Hi (0.33) value. Pusa Jaikisan and Kranti showed maximum similarity coefficient (0.81) while Varuna and Ogura were most distant (0.47), which was further confirmed by their morpho-physiological characteristics, viz. plant height, days to flowering (50%), days to maturity and 1000-seed weight. Seventeen Indian mustard genotypes were grouped into three major clusters I, II and III based on RAPD profiling. The cluster analysis was comparable up to some extent with Principal Coordinate Analysis (PCA) of two and three dimensional plots.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2012-09-17
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/23827
 
Source The Indian Journal of Agricultural Sciences; Vol 82, No 9 (2012)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/23827/11371
 
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