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De Novo Assembly of Bitter Gourd Transcriptomes: Gene Expression and Sequence Variations in Gynoecious and Monoecious Lines

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Title De Novo Assembly of Bitter Gourd Transcriptomes: Gene Expression and Sequence Variations in Gynoecious and Monoecious Lines
 
Creator Shukla, Anjali
Singh, V. K.
Bharadwaj, D. R.
Kumar, Rajesh
Rai, A.
Rai, A. K.
Mugasimangalam, R.
Parameswaran, Sriram
Singh, M.
Naik, P.S.
 
Subject Bitter gourd, Gynoecious, Monoecious, transcriptome, gene expression, sequence variation
 
Description Not Available
Bitter gourd (Momordica charantia L.) is a nutritious vegetable crop of Asian origin, used as a medicinal herb in Indian and Chinese traditional medicine. Molecular breeding in bitter gourd is in its infancy, due to limited molecular resources, particularly on functional markers for traits such as gynoecy. We performed de novo transcriptome sequencing of bitter gourd using Illumina next-generation sequencer, from root, flower buds, stem and leaf samples of gynoecious line (Gy323) and a monoecious line (DRAR1). A total of 65,540 transcripts for Gy323 and 61,490 for DRAR1 were obtained. Comparisons revealed SNP and SSR variations between these lines and, identification of gene classes. Based on available transcripts we identified 80 WRKY transcription factors, several reported in responses to biotic and abiotic stresses; 56 ARF genes which play a pivotal role in auxin-regulated gene expression and development. The data presented will be useful in both functions studies and breeding programs in bitter gourd.
Not Available
 
Date 2021-07-22T07:53:49Z
2021-07-22T07:53:49Z
2015-06-05
 
Type Research Paper
 
Identifier Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/49335
 
Language English
 
Relation Not Available;
 
Publisher PLOS ONE