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Reverse transcriptase loop-mediated isothermal amplification and reverse transcriptase recombinase amplification assays for rapid and sensitive detection of cardamom vein clearing virus

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Title Reverse transcriptase loop-mediated isothermal amplification and reverse transcriptase recombinase amplification assays for rapid and sensitive detection of cardamom vein clearing virus
Not Available
 
Creator K.P. Naveen
A. I. Bhat
 
Subject Real-time RT-PCR, RT-LAMP, RT-PCR, RT-RPA, Sensitivity
 
Description Not Available
In the present study two isothermal molecular assays viz. reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) and reverse transcriptase recombinase amplification (RT-RPA) were developed to detect the cardamom vein clearing virus (CdVCV) infecting cardamom. Assays were optimized for parameters like duration, temperature and concentration of magnesium sulfate, and betaine in the case of RT-LAMP and magnesium acetate in the case of RT-RPA. Detection limits of both assays were determined and compared with conventional RT-PCR and SYBR Green-based real-time RT-PCR. RT-LAMP was found 10000 times additional sensitive than RT-PCR and one-tenth that of real-time RT-PCR. RT-RPA was found 1000 times additional sensitive than RT-PCR and one-hundredth that of real-time RT-PCR. Both assays were specific, rapid, and sensitive for detecting CdVCV. Compared to real-time RT-PCR, these assays are economical and can be employed in large scale screening of cardamom plants against CdVCV for the selection of virus-free plants.
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Date 2021-07-28T05:17:24Z
2021-07-28T05:17:24Z
2020-05-12
 
Type Research Paper
 
Identifier Naveen KP, Bhat AI, 2020. Reverse transcriptase loop-mediated isothermal amplification and reverse transcriptase recombinase amplification assays for rapid and sensitive detection of cardamom vein clearing virus. 3 Biotech. 10, 250. https://doi.org/10.1007/s13205-020-02238-w
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http://krishi.icar.gov.in/jspui/handle/123456789/50420
 
Language English
 
Relation Not Available;
 
Publisher Springer