Reverse transcriptase loop-mediated isothermal amplification and reverse transcriptase recombinase amplification assays for rapid and sensitive detection of cardamom vein clearing virus
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Reverse transcriptase loop-mediated isothermal amplification and reverse transcriptase recombinase amplification assays for rapid and sensitive detection of cardamom vein clearing virus
Not Available |
|
Creator |
K.P. Naveen
A. I. Bhat |
|
Subject |
Real-time RT-PCR, RT-LAMP, RT-PCR, RT-RPA, Sensitivity
|
|
Description |
Not Available
In the present study two isothermal molecular assays viz. reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) and reverse transcriptase recombinase amplification (RT-RPA) were developed to detect the cardamom vein clearing virus (CdVCV) infecting cardamom. Assays were optimized for parameters like duration, temperature and concentration of magnesium sulfate, and betaine in the case of RT-LAMP and magnesium acetate in the case of RT-RPA. Detection limits of both assays were determined and compared with conventional RT-PCR and SYBR Green-based real-time RT-PCR. RT-LAMP was found 10000 times additional sensitive than RT-PCR and one-tenth that of real-time RT-PCR. RT-RPA was found 1000 times additional sensitive than RT-PCR and one-hundredth that of real-time RT-PCR. Both assays were specific, rapid, and sensitive for detecting CdVCV. Compared to real-time RT-PCR, these assays are economical and can be employed in large scale screening of cardamom plants against CdVCV for the selection of virus-free plants. Not Available |
|
Date |
2021-07-28T05:17:24Z
2021-07-28T05:17:24Z 2020-05-12 |
|
Type |
Research Paper
|
|
Identifier |
Naveen KP, Bhat AI, 2020. Reverse transcriptase loop-mediated isothermal amplification and reverse transcriptase recombinase amplification assays for rapid and sensitive detection of cardamom vein clearing virus. 3 Biotech. 10, 250. https://doi.org/10.1007/s13205-020-02238-w
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/50420 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
Springer
|
|