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Detection and partial nucleotide sequence analysis of Piper yellow mottle virus infecting black pepper (Piper nigrum L.) in India.

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Title Detection and partial nucleotide sequence analysis of Piper yellow mottle virus infecting black pepper (Piper nigrum L.) in India.
Not Available
 
Creator P.S.Hareesh
A.I.Bhat
 
Subject Badnavirus, black pepper, detection, identification, Piper yellow mottle virus, sequencing, stunted disease
 
Description Not Available
A portion of viral genome corresponding to open reading frame (ORF) I and III of the Badnavirus associated with stunted disease of black pepper (Piper nigram L.) in India was cloned and sequenced. The sequenced region of ORF I contained 694 to 710 bases while that of ORF III contained 597 to 600 bases in different isolates. Sequence analysis and phylogram confirmed that the identity of the virus as a strain of Piper yellow mottle virus (PYMoV). Further, high level of sequence conservation (=95%) was noticed in the sequenced region of both ORF I and ORF III in three isolates collected from different regions. A reliable protocol was also developed for the isolation of DNA from black pepper and PCR for detection of the virus to identify the virus-free plants of black pepper
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Date 2021-07-29T05:32:41Z
2021-07-29T05:32:41Z
2008-12-01
 
Type Research Paper
 
Identifier Hareesh, P.S. and Bhat, A.I.. 2008. Detection and partial nucleotide sequence analysis of Piper yellow mottle virus infecting black pepper (Piper nigrum L.) in India. Indian J Virol. 19: 160-167
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http://krishi.icar.gov.in/jspui/handle/123456789/50958
 
Language English
 
Relation Not Available;
 
Publisher Indian Virological Society