Detection and partial nucleotide sequence analysis of Piper yellow mottle virus infecting black pepper (Piper nigrum L.) in India.
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Title |
Detection and partial nucleotide sequence analysis of Piper yellow mottle virus infecting black pepper (Piper nigrum L.) in India.
Not Available |
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Creator |
P.S.Hareesh
A.I.Bhat |
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Subject |
Badnavirus, black pepper, detection, identification, Piper yellow mottle virus, sequencing, stunted disease
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Description |
Not Available
A portion of viral genome corresponding to open reading frame (ORF) I and III of the Badnavirus associated with stunted disease of black pepper (Piper nigram L.) in India was cloned and sequenced. The sequenced region of ORF I contained 694 to 710 bases while that of ORF III contained 597 to 600 bases in different isolates. Sequence analysis and phylogram confirmed that the identity of the virus as a strain of Piper yellow mottle virus (PYMoV). Further, high level of sequence conservation (=95%) was noticed in the sequenced region of both ORF I and ORF III in three isolates collected from different regions. A reliable protocol was also developed for the isolation of DNA from black pepper and PCR for detection of the virus to identify the virus-free plants of black pepper Not Available |
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Date |
2021-07-29T05:32:41Z
2021-07-29T05:32:41Z 2008-12-01 |
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Type |
Research Paper
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Identifier |
Hareesh, P.S. and Bhat, A.I.. 2008. Detection and partial nucleotide sequence analysis of Piper yellow mottle virus infecting black pepper (Piper nigrum L.) in India. Indian J Virol. 19: 160-167
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/50958 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Indian Virological Society
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