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Development of a single tube multiplex RT-PCR for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus associated with stunt disease of black pepper

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Title Development of a single tube multiplex RT-PCR for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus associated with stunt disease of black pepper
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Creator A. I.Bhat
S.Siju
 
Subject Black pepper, Cucumber mosaic virus, Piper yellow mottle virus, total nucleic acid isolation
 
Description Not Available
Single-tube multiplex RT–PCR was developed for the simultaneous detection of Cucumber mosaic virus and
Piper yellow mottle virus in black pepper. PCR primers designed for amplifying 650 bp from the coat protein
gene of CMV and 450 bp from the open reading frame I of PYMoV were used in the reaction. The quantity of
template used in mRT–PCR was found to be crucial for successful amplification. The method was found successful for the detection of both the viruses infecting black pepper in nursery and field samples. The method is rapid, reliable and requires only small tissue sample for sensitive detection. The entire procedure can aid in the rapid screening of a large number of plants for both viruses
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Date 2021-07-29T05:33:50Z
2021-07-29T05:33:50Z
2007-10-10
 
Type Research Paper
 
Identifier Bhat, A.I. and Siju, S. 2007. Development of a single tube multiplex RT-PCR for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus associated with stunt disease of black pepper. Current Science, 93: 973-976.
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http://krishi.icar.gov.in/jspui/handle/123456789/50963
 
Language English
 
Relation Not Available;
 
Publisher Current Science Association, Bengaluru