Development of a single tube multiplex RT-PCR for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus associated with stunt disease of black pepper
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Development of a single tube multiplex RT-PCR for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus associated with stunt disease of black pepper
Not Available |
|
Creator |
A. I.Bhat
S.Siju |
|
Subject |
Black pepper, Cucumber mosaic virus, Piper yellow mottle virus, total nucleic acid isolation
|
|
Description |
Not Available
Single-tube multiplex RT–PCR was developed for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus in black pepper. PCR primers designed for amplifying 650 bp from the coat protein gene of CMV and 450 bp from the open reading frame I of PYMoV were used in the reaction. The quantity of template used in mRT–PCR was found to be crucial for successful amplification. The method was found successful for the detection of both the viruses infecting black pepper in nursery and field samples. The method is rapid, reliable and requires only small tissue sample for sensitive detection. The entire procedure can aid in the rapid screening of a large number of plants for both viruses Not Available |
|
Date |
2021-07-29T05:33:50Z
2021-07-29T05:33:50Z 2007-10-10 |
|
Type |
Research Paper
|
|
Identifier |
Bhat, A.I. and Siju, S. 2007. Development of a single tube multiplex RT-PCR for the simultaneous detection of Cucumber mosaic virus and Piper yellow mottle virus associated with stunt disease of black pepper. Current Science, 93: 973-976.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/50963 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
Current Science Association, Bengaluru
|
|