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Detection of Fusarium wilt pathogens of Psidium guajava L. in soil using culture independent PCR (ciPCR)

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Title Detection of Fusarium wilt pathogens of Psidium guajava L. in soil using culture independent PCR (ciPCR)
Not Available
 
Creator R.K. Mishra, B.K. Pandey, Muthukumar. M., Neelam Pathak, and Mohammed Zeeshan
 
Subject Culture independent PCR; Fusarium species; Guava wilt; ITS sequences
 
Description Outreach Project
Traditional culturing methods take a long time for identification of pathogenic isolates. A protocol has been developed for the detection of Fusarium from soil samples in the early stage of infection. Seventeen soil samples from different locations were collected before the onset of rains to find out the presence of Fusarium spp. population present in the soil of guava orchards and to correlate its presence with incidence of wilt. A PCR based method was developed for the molecular characterization of Fusarium using Fusarium spp. specific primer. DNA extracted by this method was free from protein and other contaminations and the yield was sufficient for PCR amplification. The primer developed in this study was amplifying ∼230bp in all infected samples while not in healthy soil. The specificity and sensitivity of primer were tested on several Fusarium spp. and found that this primer was amplifying 10−6 dilution of the fungal DNA. The present study facilitates the rapid detection of Fusarium spp. from infected soil samples of guava collected from different agroclimatic regions in India. A rapid detection method for pathogens and a diagnostic assay for disease would facilitate an early detection of pathogen and lead to more effective control strategies.
Indian Council of Agricultural Research
 
Date 2021-08-10T10:10:06Z
2021-08-10T10:10:06Z
2012-11-10
 
Type Research Paper
 
Identifier Rupesh K. Mishra, Brajesh K. Pandey, M. Muthukumar, Neelam Pathak, Mohammad Zeeshan, Detection of Fusarium wilt pathogens of Psidium guajava L. in soil using culture independent PCR (ciPCR), Saudi Journal of Biological Sciences, Volume 20, Issue 1, 2013, Pages 51-56, https://doi.org/10.1016/j.sjbs.2012.10.007.
ISSN 1319-562X
http://krishi.icar.gov.in/jspui/handle/123456789/54256
 
Language English
 
Relation Not Available;
 
Publisher Elsevier