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Title	Molecular characterization of Steinernema abbasi from mango orchards using ITS region based markers Not Available

Creator	Kumar, H.K., Muthukumar, M., and RM Khan

Subject	nematode

Description	Not Available Soil samples were collected from the mango orchards at CISH, Rehmankhera for isolation of EPNs. A population of Steinernema sp. was extracted from soils by Galleria mellonella baiting technique (Bedding and Akhurst, 1975). The samples were purified for Koch postulates and maintained in the BOD incubator at 15 C. For molecular studies, DNA was isolated from fresh infective juveniles (one week old) by using 50 µl of worm lysis buffer (50 mM KCl, 10mm tris, 205 mm MgCl2, 0.45% NP40, 0.45% Tween 20, 0.01% gelatin and 60 µg/ml proteinase K) and homogenizing the nematodes. The suspension was incubated at 95 C for 10 min, 65 C for 1 h, and at-80 C for 2 h and finally stored in-20 C for further use. DNA was checked for its quality and quantified using UV-spectrophotometry. PCR reaction was carried out using the components in the following conditions: 10 µl of 10 X PCR buffer, 15 mM Mgcl2, 2 µl of 5 Not Available

Date	2021-08-12T06:35:11Z 2021-08-12T06:35:11Z 2016-01-05

Type	Research Paper

Identifier	Not Available http://krishi.icar.gov.in/jspui/handle/123456789/55273

Language	English

Relation	Not Available;

Publisher	The Nematological Society of India

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