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Biological Management of Banana Fusarium Wilt Caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 Using Antagonistic Fungal Isolate CSR-T-3 (Trichoderma reesei)

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Title Biological Management of Banana Fusarium Wilt Caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 Using Antagonistic Fungal Isolate CSR-T-3 (Trichoderma reesei)
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Creator Thukkaram Damodaran, Shailendra Rajan , Muthukumar Manoharan, Ram Gopal , Kavita Yadav , Sandeep Kumar , Israr Ahmad , Nidhi Kumari , Vinay K. Mishra and Sunil K. Jha
 
Subject Trichoderma reesei, banana, Fusarium wilt TR4, antifungal, LC-MS, gene expression, field evaluation
 
Description Not Available
Fusarium wilt in bananas is one of the most devastating diseases that poses a serious
threat to the banana industry globally. With no effective control measures available
to date, biological control has been explored to restrict the spread and manage the
outbreak. We studied the effective biological control potential of different Trichoderma
spp. in the management of Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4).
Expression of the defense related genes and metabolites in banana plants inoculated
with Foc TR4 and treated with effective Trichoderma sp interactions were also studied.
The in vitro growth inhibition of Foc TR4 by Trichoderma reesei isolate CSR-T-3 was
85.19% indicating a higher antagonistic potential than other Trichoderma isolates used
in the study. Further, in in vivo assays, the banana plants treated with the isolate
CSR-T-3 T. reesei had a significant reduction in the disease severity index (0.75) and also
had increased phenological indices with respect to Foc TR4 treated plants. Enhanced
activity of defense enzymes, such as β-1, 3-glucanase, peroxidase, chitinase, polyphenol
oxidase, and phenylalanine ammonia lyase with higher phenol contents were found in
the Trichoderma isolate CSR-T-3 treated banana plants challenge-inoculated with Foc
TR4. Fusarium toxins, such as fusaristatin A, fusarin C, chlamydosporal, and beauveric
acid were identified by LC-MS in Foc TR4-infected banana plants while high intensity
production of antifungal compounds, such as ß-caryophyllene, catechin-o-gallate,
soyasapogenol rhamnosyl glucoronide, peptaibols, fenigycin, iturin C19, anthocyanin,
and gallocatechin-o-gallate were detected in T. reesei isolate CSR-T-3 treated plants
previously inoculated with Foc TR4. Gene expression analysis indicated the upregulation
of TrCBH1/TrCBH2, TrXYL1, TrEGL1, TrTMK1, TrTGA1, and TrVEL1 genes in CSR-T-3
treatment. LC-MS and gene expression analysis could ascertain the upregulation of
genes involved in mycoparasitism and the signal transduction pathway leading to
secondary metabolite production under CSR-T-3 treatment.
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Date 2021-08-12T06:27:15Z
2021-08-12T06:27:15Z
2020-12-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/55261
 
Language English
 
Relation Not Available;
 
Publisher Not Available