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Plant growth promoting, biochemical and antifungal properties of microbes isolated from cow dung

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Title Plant growth promoting, biochemical and antifungal properties of microbes isolated from cow dung
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Creator R.A. Ram, Govind Kumar, Shailendra Kumar Maurya, S. Rajan and Israr Ahmad
 
Subject Microbial population, Azotobacter, P-solubilising bacteria, Bio-chemical test, Plant growth pro- moting activity
 
Description Not Available
Cow dung is a rich source of microbial diversity and being used since long time in India for seed treatment,
plastering cut ends of vegetative propagated plant parts and sprinkling of diluted solution on crops. In pres-
ent study, microbial isolation from fresh cow (indigenous) dung observed in different generalized and selective
medium. Cow dung enriched with diversity of microorganisms as it contained various microbial population as
observed in different mediumi.e38 x 106cfu g-1 (in nutrient agar), 26.0 x 106 cfu g-1 (actinomycetes isolation agar), 36 x 106 cfu g-1 (Kings B agar), 16 x 106 cfu g-1 (CV agar),13 x 106 cfu g-1 (Methyl red), 15 x 106 cfu g-1 (Pikovskyas agar), 30 x 106 cfu g-1 (CRYEMA), Azospirillum 70 x 106 cfu g-1 (N-free maleate) and 30 x 106
cfu g-1
(Jenson agar medium). Based on microbial enumeration, total 18 bacterial isolates were selected for biochemical, PGPR characterization and biocontrol activity. All isolates were tested positive for phosphate and Zn solubilisation. Isolate CD2, CD5, CD13, CD14 and CD19 were tested highly positive for zinc, phosphate, K-solubilisation, IAA and amylase pro-duction. Test isolates CD2, CD5, CD6, CD7, CD10 and CD13 were tested highly positive for β-galactosidase activ-ity, ornithine utilization, nitrate reduction, citrate utilization, malonate utilization, esculin hydrolysis, trehalose, glucose and lactose utilization. All isolated microbes tested for biocontrol activity (antifungal property) against
standard pathogen procured from microbial type culture collection (MTCC), i.e. C. gloeosporioides (MTCC 2190),
F. oxysporum (MTCC 10247), C. fimbriota (MTCC 2281) and P. aphanidermatum (MTCC 284). CD2, CD34 and CD20
isolates significantly inhibited the growth of C. gloeosporioides and P. aphanidermatum.
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Date 2021-08-12T07:59:38Z
2021-08-12T07:59:38Z
2020-12-01
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/55301
 
Language English
 
Relation Not Available;
 
Publisher Not Available