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cpSSR marker based DNA fingerprinting and diversity assessment of mango genotypes

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Title cpSSR marker based DNA fingerprinting and diversity assessment of mango genotypes
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Creator Ahmad Israr, Bajpai Anju and Rajan Shailendra
 
Subject cpSSR, mango genotypes, genetic diversity
 
Description Not Available
Germplasm characterization is a pre-requisite for their
conservation and utilization in breeding programme.
We used cpSSR markers for the first time to identify a
set of polymorphic microsatellite loci and analysis of
genetic diversity within mango genotypes. The present
study was aimed to assess the intraspecific
relationships among 28 diverse mango genotypes using
uniparental and haploid chloroplast SSR (cpSSR)
marker. A total of 27 alleles were identified with an
average of 1.5 allele per locus. Seven out of 18 primer
pairs showed polymorphic bands with a number of
alleles ranging from one to three. The genotypes
studied revealed significant levels of cpDNA genetic
diversity. Shannon diversity index and gene diversity
varied, from 0.07-0.32 and 0.34-0.95 respectively.
Marker index and resolving power were ranged 0.04-
2.14 and 0.07-1.71. cpSSR markers are suitable for characterization and
assessment across diverse mango genotypes and there
is small difference in the genetic diversity with the
species. Results indicated that two mango cultivars
(Sukul and Himsagar) were found to be far distant of
the genetic relationship from the other cultivars.
Analysis of overall diversity of mango genotypes
revealed the less intraspecies diversity (40%). This low
intraspecific diversity is due to high degree of gene flow
in population through random mating without barrier.
The information of this work will be useful for selection
and more efficient utilization of mango germplasm in
breeding programs in the future.
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Date 2021-08-12T10:00:43Z
2021-08-12T10:00:43Z
2018-12-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/55403
 
Language English
 
Relation Not Available;
 
Publisher Not Available