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Characterization of novel French bean leaf curl virus and beta satellite associated with leaf curl disease of French bean (Phaseolus vulgaris L.),

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Title Characterization of novel French bean leaf curl virus and beta satellite associated with leaf curl disease of French bean (Phaseolus vulgaris L.),
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Creator Venkataravanappa, V., Priti Sonavane, Lakshminarayana Reddy, C. N., Reddy, M. K.,
 
Subject Begomovirus · Recombination · Phylogenetic analysis · Polymerase chain reaction · Betasatellite
 
Description Not Available
Leaves from French bean plants with severe leaf curl (ten samples) and from asymptomatic plants from 10 felds (FB1–10)
in the Western Ghat region of Karnataka, India were collected. Virus isolate FB01 from French bean was shown to be transmitted by whitefy Bemisia tabaci cryptic species Asia I. DNA was isolated from the samples (ten feld samples and one
sample infected with FB01 via whitefy) and subjected to PCR to confrm begomovirus infection using specifc primers. All
11 were positive for the expected 1.2-kb amplicon. The sequence analysis of the 1.2-kb amplicon showed that all isolates
shared more than 98% nucleotide identity. Further, the complete genome from isolate FB01 was amplifed and sequenced
and shown to share a maximum nucleotide identity (89.3%) with Ageratum yellow vein Sri Lanka virus. As per the ICTV
species threshold for begomoviruses, the virus is considered as a new species, with the proposed name French bean leaf curl
virus (FbLCV). The samples were subjected to PCR using specifc primers for DNA B, betasatellite and alphasatellite; only
betasatellites were amplifed. The detected betasatellite shared maximum nucleotide identity (92.6%) with Synedrella yellow
vein betasatellite. Recombination analysis indicated that FbLCV and betasatellite were recombinants. The signifcance of
these fndings are discussed.
Indian Council of Agricultural Research
 
Date 2021-08-05T08:33:13Z
2021-08-05T08:33:13Z
2020-07-01
 
Type Research Paper
 
Identifier Venkataravanappa, V., Priti Sonavane, Lakshminarayana Reddy, C. N., Reddy, M. K., 2020, Characterization of novel French bean leaf curl virus and beta satellite associated with leaf curl disease of French bean (Phaseolus vulgaris L.), Journal of General Plant Pathology, https://doi.org/10.1007/s10327-020-00979-3
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http://krishi.icar.gov.in/jspui/handle/123456789/53267
 
Language English
 
Relation Not Available;
 
Publisher Journal of General Plant Pathology