Record Details


Field	Value

Title	Micropropagation of guava Not Available

Creator	Mishra,M., Chandra,R.,Pati,R. and Bajpai,A.

Subject	shoot bud culture, proliferation, MS media

Description	Not Available A foolproof method of micropropagation through in vitro shoot bud culture has been developed in guava cv. ‘Allahabad Safeda’. The stock plant of guava was maintained at shade net house (75% shade) and pruned severely to encourage new growth. Around 3 cm long shoots were excised and defoliated. The explants were pretreated in solution containing 0.1% Carbendazime and 100 mg/L PVP for 1 hour and then washed with Tween-20 wetting agent. The pretreated explants were further treated with HgCl2 0.1% for 5 minutes aseptically followed by six washing in autoclaved distilled water. The sterilized explants were cultured on MS medium supplemented with BAP (3.0 mg/L) for shoot bud induction and proliferation. The proliferated shoots were then subcultured on MS medium containing 10 mg/L IBA for rhizogenesis. The rooted plants were finally shifted to autoclaved coconut husk fortified with ½ MS salt mixture for acclimatization. Acclimatized plantlets of guava cv. Allahabad Safeda were planted. Not Available

Date	2021-08-13T06:06:21Z 2021-08-13T06:06:21Z 2007-01-01

Type	Article

Identifier	Not Available Not Available http://krishi.icar.gov.in/jspui/handle/123456789/56222

Language	English

Relation	Not Available;

Publisher	Not Available

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