Record Details

Development of high conserved cross-species microsatellite markers from cucumber genome and their applicability in genetic diversity and comparative mapping

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Development of high conserved cross-species microsatellite markers from cucumber genome and their applicability in genetic diversity and comparative mapping
Not Available
 
Creator Sudhakar Pandey
Punam Singh Yadav
Waquar Akhter Ansari
Maneesh Pandey
Luming Yang
Bijendra Singh
Rakesh Kumar Dubey
Prabhakar Mohan Singh
Jagdish Singh
 
Subject Simple sequence repeats marker Cucurbit species Transferability Polymorphism
 
Description Not Available
One of the most widely applicable and used markers considered in plant research is simple sequence repeats (SSRs). The Gy14 cucumber genome provided information for the development of SSR marker in this species which might be used in other related species additionally. We, therefore, developed genome-wide SSR markers from cucumber, which were used for genetic diversity analysis in 16 different cucurbits species and comparative mapping with melon and watermelon. A total of 2171 SSR motifs were identified from seven cucumber chromosomes. Out of them, 1749 markers belong to melon while 442 markers to watermelon. We experimentally validated 70 SSR markers to check their cross-species transferability among 16 different cucurbits species belongs to six genera. Maximum% polymorphism has been recorded for Momordica charantia (82%) followed by Momordica cochinchinesis (78.18%) and Luffa harmaphrodita (73.68%) while Benincasa hispida (49.21%) exhibited minimum% polymorphism. The large number of cucumber SSR markers developed in the present study provides a valuable resource for genetic linkage map construction, molecular mapping, and marker-assisted selection (MAS) in cucumber. Furthermore, the cross-species transferable SSR markers could also be useful in various molecular markers related studies in other closely related species in the Cucurbitaceae family in which draft genomes are not yet available.
Not Available
 
Date 2021-08-04T10:19:06Z
2021-08-04T10:19:06Z
2021-07-01
 
Type Research Paper
 
Identifier Not Available
0304-4238r
http://krishi.icar.gov.in/jspui/handle/123456789/53076
 
Language English
 
Relation Not Available;
 
Publisher Scientia Horticulturae