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An Agrobacterium mediated genetic transformation of guava (Psidium guajava L.) with endochitinase gene

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Title An Agrobacterium mediated genetic transformation of guava (Psidium guajava L.) with endochitinase gene
Not Available
 
Creator Mishra, M., Jalil, U.,Sharma, N. and Hudedamani, U
 
Subject Genetic transformation, Psidium guajava, endochitinase, wilt disease
 
Description Not Available
Genetic transformation of guava (Psidium guajava L.) was developed for the first time using in vitro grown shoot tip explant co-
cultivated with Agrobacterium tumefaciens strain LBA4404 harbouring binary vector pIIHR-JBMch with endochitinase and nptII genes.
The highest transformation efficiency was achieved by wounding explants with tungsten particles (0.5 µm) through particle acceleration
system, followed by infection for 45 minutes with A. tumefaciens, grown overnight with 100 µM acetosyringone, corresponding to
OD600
=0.5 followed by co-cultivation for 72 hours under dark condition on co-cultivation medium (MS+100 µM acetosyringone+100 mg
L-1
L-Cystein). Putative transformed explants regenerated shoots on selection medium stressed with 200 mg L-1
kanamycin for 12
weeks. Molecular analysis of putative transformants by PCR confirmed the integration of endochitinase and nptII gene in the plant
nuclear genome.
Not Available
 
Date 2021-08-13T06:42:45Z
2021-08-13T06:42:45Z
2014-01-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/56250
 
Language English
 
Relation Not Available;
 
Publisher Not Available