In vitro screening of guava transformed with endochitinase gene against Fusarium oxysporum psidii
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
In vitro screening of guava transformed with endochitinase gene against Fusarium oxysporum psidii
Not Available |
|
Creator |
Mishra, M., Jalil, S.U. , Mishra,R.K. Kumari, S and Pandey, B.K.
|
|
Subject |
chitinase; genetic transformation; pathogen inhibition assay; quantification of chitinase; wilt disease
|
|
Description |
Not Available
Guava wilt disease is a severe threat to guava growers all over the world. It is caused by the soil-borne fungus Fusarium oxysporum f.sp. psidii. To control the disease, the Trichoderma-endochitinase gene was first introgressed into guava (Psidium guajava L.). The transgenic plantlets were screened in vitro for resistance against the wilt pathogen. Six-months-old genetically transformed plants raised in cocopeat under in vitro conditions were inoculated with a 7-days old culture of F. oxysporum f.sp. psidii. The presence of the pathogen in the cocopeat medium was confirmed by cultural as well as PCR analysis using species-specific primers. The roots of transgenic plants were wounded to facilitate the entry of the pathogen. The histopathological analysis revealed the presence of mycelium in vascular bundles. However, none of the plants showed symptoms of wilt disease during the investigation. In vitro pathogen inhibition assay and subsequently spore germination assay revealed that the crude leaf extract of transformed plants inhibited the germination of fungal conidia. The leaf tissue studied for expression of endochitinase revealed that two transgenic plants showed very high activity of N-acetyld-glucosamine (0.741 and 0.738 µM/min/µg of protein, respectively) which clearly indicated that transgenic plants could not develop any symptoms of wilt disease due to overexpression of endochitinase. Not Available |
|
Date |
2021-08-13T08:00:00Z
2021-08-13T08:00:00Z 2016-01-01 |
|
Type |
Article
|
|
Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/56262 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
Not Available
|
|