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In vitro screening of guava transformed with endochitinase gene against Fusarium oxysporum psidii

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Title In vitro screening of guava transformed with endochitinase gene against Fusarium oxysporum psidii
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Creator Mishra, M., Jalil, S.U. , Mishra,R.K. Kumari, S and Pandey, B.K.
 
Subject chitinase; genetic transformation; pathogen inhibition assay; quantification of chitinase; wilt disease
 
Description Not Available
Guava wilt disease is a severe threat to guava growers all over the world. It is caused by the soil-borne fungus
Fusarium oxysporum f.sp. psidii. To control the disease, the Trichoderma-endochitinase gene was first introgressed into guava (Psidium guajava L.). The transgenic plantlets were screened in vitro for resistance against
the wilt pathogen. Six-months-old genetically transformed plants raised in cocopeat under in vitro conditions
were inoculated with a 7-days old culture of F. oxysporum f.sp. psidii. The presence of the pathogen in the cocopeat medium was confirmed by cultural as well as PCR analysis using species-specific primers. The roots of
transgenic plants were wounded to facilitate the entry of the pathogen. The histopathological analysis revealed
the presence of mycelium in vascular bundles. However, none of the plants showed symptoms of wilt disease
during the investigation. In vitro pathogen inhibition assay and subsequently spore germination assay revealed
that the crude leaf extract of transformed plants inhibited the germination of fungal conidia. The leaf tissue
studied for expression of endochitinase revealed that two transgenic plants showed very high activity of N-acetyld-glucosamine (0.741 and 0.738 µM/min/µg of protein, respectively) which clearly indicated that transgenic
plants could not develop any symptoms of wilt disease due to overexpression of endochitinase.
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Date 2021-08-13T08:00:00Z
2021-08-13T08:00:00Z
2016-01-01
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/56262
 
Language English
 
Relation Not Available;
 
Publisher Not Available