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A highly reliable DNA marker in dioecious plant Japanese hop (Humulus scandens)

Indian Agricultural Research Journals

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Title A highly reliable DNA marker in dioecious plant Japanese hop (Humulus scandens)
 
Creator GAO, WUJUN
JI, YANKE
DENG, CHUANLIANG
LU, LONGDOU
 
Subject —
 
Description One hundred decamer primers of random amplified polymorphic DNA were tested on dioecious Humulus scandens plants to analysis genome difference. One of the 100 primers, S2142, produced one male-linked band (S2142₇₆₂), with the length of 762bp, rich in AT (54.7%). The marker was sequenced and specific primer was constructed to generate correspondingly size marker of sequence characterized amplified regions (SCAR) in male plants, SCAR₇₆₂, which is suitable for a precise, rapid identification of male plants in 30 male and female H. scandens plants, respectively. Southern hybridization, using S2142₇₆₂ as a probe, showed that S2142₇₆₂ exists in the male genomes, but not in the female genome. This result strongly suggests that S2142₇₆₂ is located on the chromosome region that is specific to the male.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2011-08-03
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/8403
 
Source The Indian Journal of Agricultural Sciences; Vol 81, No 8 (2011)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/8403/3390
 
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