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Molecular cloning and nutritional regulation of putative∆6 desaturase mRNA from striped catfish (Pangasianodon hypophthalmus)
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| Title |
Molecular cloning and nutritional regulation of putative∆6 desaturase mRNA from striped catfish (Pangasianodon hypophthalmus)
Not Available |
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| Creator |
Avinash Rasal, Surva Ror, R S Rana, S Murli, Gopal Krishna, Subodh Gupta, P Gireesh-Babu
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| Subject |
Δ6 fads Striped catfish Pangasius cDNA Fatty acid Gene regulation
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| Description |
Not Available
Here we report the full-length cDNA cloning and nutritional regulation of a putative Δ6 fatty acyl desaturase (Δ6 fads) gene from striped catfish, Pangasianodon hypophthalmus. The 5′ and 3′ RACE analysis revealed a cDNA sequence of 1896 base pairs (bp) comprising a 5′ UTR of 49 bp, 3′ UTR of 509 bp and an open reading frame (ORF) of 1338 bp specifying a protein of 445 amino acids. Bioinformatics analysis showed that the deduced peptide sequence included all the characteristic features of microsomal fatty acyl desaturases, including N-terminal cytochrome b5 domain containing the haem binding motif H-P-G-G, fatty acyl (FA) desaturase domain, three histidine boxes and transmembrane regions. P. hypophthalmus ∆6 desaturase peptide sequence shares high similarity with Pangasius larnaudii (95%) and Clarias macrocephalus (90%). The predicted secondary and tertiary protein structures revealed that desaturase protein contains mostly α-helix followed by random coils and strands. Tissue distribution analysis showed that Δ6 fads is mainly expressed in brain followed by liver, heart, gill and muscle. Since α-linolenic acid is a substrate for the Δ6 FADS enzyme, various concentrations of this fatty acid (0.5, 1, 1.5 and 2 g kg− 1) were administered to experimental fish through feed to find out its effect on the expression of Δ6 fads. Results showed that the liver Δ6 fads gene expression is up-regulated in fish fed with experimental diets (p < 0.05). Not Available |
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| Date |
2016-11-30T10:43:32Z
2016-11-30T10:43:32Z 2016-01-01 |
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| Type |
Research Paper
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| Identifier |
1
http://dx.doi.org/10.1016/j.aquaculture.2015.10.003 http://krishi.icar.gov.in/jspui/handle/123456789/746 |
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| Language |
English
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| Relation |
Not Available;
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| Publisher |
Elsevier
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